Search
Applied Biosystems™
MagMAX™ mirVana™ RNA Isolation Kits
The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types.
| Número de catálogo | ProductTypeSpecs | Cantidad |
|---|---|---|
| A27828 | 96 reacciones |
Número de catálogo A27828
Precio (EUR)
768,00
Each
Cantidad:
96 reacciones
Precio (EUR)
768,00
Each
Scalable isolation of RNA (including small RNAs)
The MagMAX™ mirVana™ Total RNA Isolation Kit is designed for isolation of total RNA, including small RNAs such as microRNAs, from a wide variety of sample types. The kit uses MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.
The magnetic bead–based purification format allows you to easily scale from processing 6 to 96 samples at once when used with the KingFisher™ Duo Prime or KingFisher™ Flex Magnetic Particle Processor. Alternatively, samples can be processed manually with the use of a magnetic stand.
Features of the MagMAX mirVana Total RNA Isolation Kit include:
- Automation-ready, phenol-free extraction
- Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
- Recovery of pure miRNAs compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and TaqMan™ Advanced miRNA Assays
One kit to isolate true total RNA from a diverse set of sample types
Why purchase multiple purification kits to isolate all RNAs present in your different sample types? The MagMAX mirVana Total RNA Isolation Kit eliminates the headache of ordering 'specialized' kits because it has been optimized for use with most biological samples. No longer are separate, specific kits needed to isolate microRNAs from plasma and solid tissue samples.
The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells from small RNAs such as microRNAs to non-coding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic-bead format makes the kit fully amenable to automated RNA isolation from the following samples:
- Serum/plasma
- Whole blood
- Tissue
- Cell culture
- Urine
Benefits of MagMAX magnetic bead–based purification
Magnetic beads offer many benefits compared to other technologies for isolating total RNA. Beads bind RNA more efficiently than glass fiber filters, thereby enabling higher and more consistent yields. Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Volumen de elución20 to 100 μL
Tipo de producto finalARN total
Para utilizar con (aplicación)Secuenciación de última generación, PCR en tiempo real, análisis de microARN
Para utilizar con (equipo)KingFisher™ Duo Prime, KingFisher™ Flex, KingFisher™ Apex
Compatibilidad de alto rendimientoCompatible con alto rendimiento
Tiempo de purificaciónUp to 1.5 hr.
Cantidad96 reacciones
Condiciones de envíoHielo seco
Cantidad de material de partidaCells: ≤106
Plasma: ≤100 μL
Serum: ≤100 μL
Tissue: ≤50 mg
Urine: ≤250 μL
Whole Blood: ≤50 μL
Plasma: ≤100 μL
Serum: ≤100 μL
Tissue: ≤50 mg
Urine: ≤250 μL
Whole Blood: ≤50 μL
ProducciónCells: ≤32 μg RNA per 50,000 cells
Tissue: ≤500 μg per 5 mg
Tissue: ≤500 μg per 5 mg
Isolation TechnologyMagnetic Bead
Tipo de muestraSangre, células, Muestras líquidas (p. ej., suero), ARN, tejido, Whole Blood
TargetARN total, ARNm, ARNip
Unit SizeEach
Contenido y almacenamiento
Caja 1 (almacenar a entre -15 y -25 °C)
• 1 x 0,48 ml de proteinasa K, 50 mg/ml
• 1 x 0,96 ml de potenciador de solución de lisis o solución vinculante
• 1 x 0,2 ml de ADNasa TURBO™, 20 U/UL
Caja 2 (almacenar a temperatura ambiente)
• 1 x 115 ml de tampón de lisis
• 1 x 4,4 ml de tampón de digestión PK
• 1 x 2 ml de gránulos de unión a ARN
• 1 x 20 ml de solución de lavado concentrado 1
• 1 x 60 ml de solución de lavado 2 concentrado
• 1 x 4,8 ml de tampón de reunión
• 1 x 4,6 ml de tampón de ADNasa TURBO™ MagMAX™
• 1 x 9,6 ml de tampón de elución
• 1 placa de procesamiento
• 2 placas de elución
• 4 cubiertas de placas