MQAE (N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide)
MQAE (<i>N</i>-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide)
Citas y referencias (86)
Invitrogen™

MQAE (N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide)

MQAE es un derivado de 6-metoxiquinolina y es un indicador fluorescente para el Cl intracelular-. Este tinte detecta el iónMás información
Have Questions?
Número de catálogoCantidad
E3101100 mg
Número de catálogo E3101
Precio (EUR)
434,00
Each
Añadir al carro de la compra
Cantidad:
100 mg
Precio (EUR)
434,00
Each
Añadir al carro de la compra
MQAE es un derivado de 6-metoxiquinolina y es un indicador fluorescente para el Cl intracelular-. Este tinte detecta el ión a través de supresión por colisión limitada por difusión MQAE tiene mayor sensibilidad a CL- y una mayor producción cuántica de fluorescencia que SPQ; por lo tanto, actualmente es el más utilizado de los dos indicadores.

Obtenga más información sobre los indicadores de iones, incluidos el calcio, el potasio, el pH y los indicadores de potencial de membrana ›

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteA base de colorantes fluorescentes
Cantidad100 mg
Condiciones de envíoTemperatura ambiente
Para utilizar con (aplicación)Viabilidad y proliferación celulares
Para utilizar con (equipo)Microscopio confocal, Citómetro de flujo
Tipo de productoMQAE
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Citations & References (86)

Citations & References
Abstract
Noninvasive measurement of chloride concentration in rat olfactory receptor cells with use of a fluorescent dye.
Authors:Kaneko H, Nakamura T, Lindemann B
Journal:Am J Physiol Cell Physiol
PubMed ID:11350733
Inwardly directed Ca(2+)-dependent chloride currents are thought to prolong and boost the odorant-induced transient receptor currents in olfactory cilia. Cl(-) inward current, of course, requires a sufficiently high intracellular Cl(-) concentration ([Cl(-)](i)). In previous measurements using a fluorescent Cl(-) probe, N-(ethoxycarbonylmethyl)-6-methoxyquinolinium bromide (MQAE), [Cl(-)](i) of newt olfactory cells was estimated ... More
TRPV1 acts as proton channel to induce acidification in nociceptive neurons.
Authors:Hellwig N, Plant TD, Janson W, Schäfer M, Schultz G, Schaefer M
Journal:J Biol Chem
PubMed ID:15173182
The low extracellular pH of inflamed or ischemic tissues enhances painful sensations by sensitizing and activating the vanilloid receptor 1 (TRPV1). We report here that activation of TRPV1 results in a marked intracellular acidification in nociceptive dorsal root ganglion neurons and in a heterologous expression system. A characterization of the ... More
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Authors:Driessen AJ, van den Hooven HW, Kuiper W, van de Kamp M, Sahl HG, Konings RN, Konings WN
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent ... More
Determination of the intracellular free chloride concentration in rat brain synaptoneurosomes using a chloride-sensitive fluorescent indicator.
Authors:Engblom AC, Akerman KE
Journal:Biochim Biophys Acta
PubMed ID:8274496
'The chloride-sensitive fluorescent indicator MQAE (N-(6-methoxyquinolyl) acetoacetyl ester) has been used for determination of the intracellular free chloride concentration in rat brain synaptoneurosomes. Loading of the synaptoneurosomes with MQAE occurs by transmembrane diffusion. Calibration of the intracellular MQAE was done by determining the correlation between fluorescence intensity and intrasynaptoneurosomal Cl- ... More
Effects of sasanquasaponin on ischemia and reperfusion injury in mouse hearts.
Authors:Lai ZF, Shao Z, Chen YZ, He M, Huang Q, Nishi K
Journal:J Pharmacol Sci
PubMed ID:15037817
'We investigated effects of sasanquasaponin (SQS), a traditional Chinese herb''s effective component, on ischemia and reperfusion injury in mouse hearts and the possible role of intracellular Cl- homeostasis on SQS''s protective effects during ischemia and reperfusion. An in vivo experimental ischemia model was made in mice (weight 27-45 g) using ... More
View all MQAE (N-(Ethoxycarbonylmethyl)-6-Methoxyquinolinium Bromide) citations