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5-Bromo-2'-desoxiuridina, 99 %, Thermo Scientific Chemicals
5-Bromo-2'-desoxiuridina, 99 %, Thermo Scientific Chemicals
5-Bromo-2'-desoxiuridina, 99 %, Thermo Scientific Chemicals
Thermo Scientific Chemicals

5-Bromo-2'-desoxiuridina, 99 %, Thermo Scientific Chemicals

CAS: 59-14-3 | C9H11BrN2O5 | 307.10 g/mol
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Cantidad:
250 mg
1 g
5 g
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Número de catálogo H27260.06
también denominado H27260-06
Precio (EUR)
385,00
Each
Cantidad:
5 g
Pedido a granel o personalizado
Precio (EUR)
385,00
Each
Identificadores químicos
CAS59-14-3
IUPAC Name5-bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,2,3,4-tetrahydropyrimidine-2,4-dione
Molecular FormulaC9H11BrN2O5
InChI KeyWOVKYSAHUYNSMH-RRKCRQDMSA-N
SMILESOC[C@H]1O[C@H](C[C@@H]1O)N1C=C(Br)C(=O)NC1=O
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EspecificacionesSpecification SheetHoja de especificaciones
Appearance (Color)White to pale cream
Assay (HPLC)≥98.5%
Optical Rotation+31.0 ± 1.5? (c=1 in 0.1N NaOH) or +23.0 ± 1? (c=1 in water)
FormCrystals or powder or crystalline powder
Identification (FTIR)Conforms (UK sourced product only)
5-Bromo-2'-deoxyuridine (BrdU) is a thymidine analog used to label DNA. It is incorporated into newly synthesized DNA in place of thymidine during the S phase of the cell cycle. Cells that were actively proliferating can then be detected by denaturing the DNA and allowing specific antibodies to target the BrdU incorporation. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Aplicaciones
La 5-bromo-2′-desoxiuridina (BrdU) es un análogo de la timidina utilizado para etiquetar el ADN. Se incorpora al ADN recién sintetizado en lugar de la timidina durante la fase S del ciclo celular. Entonces las células que proliferaban activamente pueden detectarse al desnaturalizar el ADN y permitir que determinados anticuerpos se dirijan a la incorporación de BrdU. En consecuencia, la 5-BrdU se utiliza para estudiar la señalización celular y otros procesos que inducen a la proliferación celular.

Solubilidad
Soluble en agua.

Notas
Almacenar en un lugar fresco. Mantener el recipiente bien cerrado en un lugar seco y bien ventilado. Almacenar lejos de los agentes oxidantes fuertes.
RUO – Research Use Only

General References:

  1. Chen Li,; Avrum I. Gotlieb. Transforming Growth Factor-β Regulates the Growth of Valve Interstitial Cellsin Vitro. The American Journal of Pathology. 2011, 179 (4), 1746-1755 .
  2. Yu Gui et. al. Signaling mechanisms mediating uridine adenosine tetraphosphate-induced proliferation of human vascular smooth muscle cells. Journal of Cardiovascular Pharmacology. 2011 , 58(6), 654-662 .
  3. Thymidine analog, preferentially incorporated into cellular DNA in place of thymidine, producing increased radiosensitivity of the cell: Int. J. Radiat. Oncol. Biol. Phys., 10, 1399 (1984); 21, 709 (1991). Mutagen used in genetic research: Mutat. Res., 258, 161 (1991). Reviews on use in diagnostics and research: Histochem. J., 27, 339, 923 (1995; 28, 531 (1996).