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Citas y referencias (10)
Invitrogen™
Hexidium Iodide
La tinción de ácido nucleico de yoduro de hexidio es permeable a las células de mamíferos y tiñe de formaMás información
| Número de catálogo | Cantidad |
|---|---|
| H7593 | 5 mg |
Número de catálogo H7593
Precio (EUR)
450,00
Each
Cantidad:
5 mg
Precio (EUR)
450,00
Each
La tinción de ácido nucleico de yoduro de hexidio es permeable a las células de mamíferos y tiñe de forma selectiva casi la mayoría de bacterias grampositivas en presencia de bacterias gramnegativas. El yoduro de hexidio muestra un nivel máximo de excitación/emisión de ∼518/600 nm al enlazarse con el ADN. Generalmente, tanto el citoplasma como los núcleos de las células eucariotas muestran tinción con yoduro de hexidio.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorNaranja
Método de detecciónFluorescente
Tipo de colorantePermeabilidad celular
Intervalo de longitud de onda de excitación518⁄600
Para utilizar con (equipo)Microscopio de fluorescencia
Cantidad5 mg
Condiciones de envíoTemperatura ambiente
Tipo de etiquetaFluorescent Dye
Tipo de productoYoduro de hexidio
SubCellular LocalizationNúcleo, citoplasma y citosol, Cytosol, Nucleus
Unit SizeEach
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Citations & References (10)
Citations & References
Abstract
A flow-cytometric gram-staining technique for milk-associated bacteria.
Journal:Appl Environ Microbiol
PubMed ID:12732558
'A Gram-staining technique combining staining with two fluorescent stains, Oregon Green-conjugated wheat germ agglutinin (WGA) and hexidium iodide (HI) followed by flow-cytometric detection is described. WGA stains gram-positive bacteria while HI binds to the DNA of all bacteria after permeabilization by EDTA and incubation at 50 degrees C for 15
A fluorescent Gram stain for flow cytometry and epifluorescence microscopy.
Journal:Appl Environ Microbiol
PubMed ID:9647848
'The fluorescent nucleic acid binding dyes hexidium iodide (HI) and SYTO 13 were used in combination as a Gram stain for unfixed organisms in suspension. HI penetrated gram-positive but not gram-negative organisms, whereas SYTO 13 penetrated both. When the dyes were used together, gram-negative organisms were rendered green fluorescent by
Metabolic activity in filamentous fungi can be analysed by flow cytometry.
Journal:J Microbiol Methods
PubMed ID:12782375
The use of flow cytometry in combination with fluorescent dyes as a technique to rapidly differentiate and enumerate bacterial and yeast cells is well established. We have shown that through the judicial choice of stains, the nondestructive screening and sorting of fungal material is possible. The early stages of growth,
Increased sensitivity of bacterial detection in cerebrospinal fluid by fluorescent staining on low-fluorescence membrane filters.
Journal:J Med Microbiol
PubMed ID:16091435
A membrane-filter-based, fluorescent Gram stain method for bacterial detection in cerebrospinal fluid samples was developed and evaluated as a rapid, sensitive alternative to standard Gram stain protocols. A recently developed, modified version of the aluminium oxide membrane Anopore with low-fluorescence optical properties showed superior performance in this application. Other aspects
Nucleic acid stains as indicators of Cryptosporidium parvum oocyst viability.
Journal:Int J Parasitol
PubMed ID:9279581
We developed nucleic acid dye staining methodology for untreated, heat-treated and chemically inactivated C. parvum oocysts. The nucleic acid staining was compared to in vitro excystation and animal infectivity using split samples of oocysts. Among the nucleic acid stains tested, SYTO-9, hexidium and SYTO-59 stained the oocysts consistently, and the