LysoTracker™ Green DND-26, envase especial

Citas y referencias (102)

Invitrogen™

LysoTracker™ Green DND-26, envase especial

DND-26 LysoTracker™ Green es un colorante verde fluorescente que tiñe los compartimentos ácidos de las células vivas con unos nivelesMás información

Número de catálogo	Cantidad
L7526	20 x 50 μL

Número de catálogo L7526

Precio (EUR)

674,00

Añadir al carro de la compra

Cantidad:

20 x 50 μL

Precio (EUR)

674,00

Añadir al carro de la compra

DND-26 LysoTracker™ Green es un colorante verde fluorescente que tiñe los compartimentos ácidos de las células vivas con unos niveles máximos de excitación/emisión de ∼504/511 nm.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorVerde

Concentración1 mM

DescripciónLysoTracker™ Green DND-26

Método de detecciónFluorescente

EmisiónGreen

Intervalo de longitud de onda de excitación504⁄511

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosLysoTracker

Cantidad20 x 50 μL

Condiciones de envíoTemperatura ambiente

Tipo de etiquetaFluorescent Dye

Tipo de productoTinte

SubCellular LocalizationLisosomas

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

What is the recommended incubation time for LysoTracker Green DND-26 (Cat. No. L7526) and LysoSensor Yellow/Blue DND-160 (Cat. No. L7545)?

LysoTracker Green DND-26 (Cat. No. L7526) and LysoSensor Yellow/Blue DND-160 (Cat. No. L7545) show faster uptake in cells and consequently a faster "alkalizing effect" on the lysosomes, so we recommend incubating the cells with these probes for 1-5 min at 37 degrees C. As the other LysoTracker and LysoSensor probes have a slower rate of uptake, we recommend a longer incubation time of 30 min to 2 hr.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (102)

Citations & References

Abstract

Biocompatibility, endocytosis, and intracellular trafficking of mesoporous silica and polystyrene nanoparticles in ovarian cancer cells: effects of size and surface charge groups.

Authors:Ekkapongpisit M, Giovia A, Follo C, Caputo G, Isidoro C,

Journal:Int J Nanomedicine

PubMed ID:22904626

Nanoparticles engineered to carry both a chemotherapeutic drug and a sensitive imaging probe are valid tools for early detection of cancer cells and to monitor the cytotoxic effects of anticancer treatment simultaneously. Here we report on the effect of size (10-30 nm versus 50 nm), type of material (mesoporous silica ... More

Induction of autophagy and cell death by tamoxifen in cultured retinal pigment epithelial and photoreceptor cells.

Authors:Cho KS, Yoon YH, Choi JA, Lee SJ, Koh JY,

Journal:Invest Ophthalmol Vis Sci

PubMed ID:22786900

We investigated the mechanism of tamoxifen (TAM) retinotoxicity using human retinal pigment epithelial (RPE)-derived (ARPE-19) and photoreceptor-derived (661W) cells. Cultured ARPE-19 and 661W cells were treated with 5 to 10 ÂµM TAM, and the resultant cell death was quantified using lactate dehydrogenase (LDH) release assay. Cellular oxidative stress was determined ... More

Regulation of endocytosis via the oxygen-sensing pathway.

Authors:Wang Y, Roche O, Yan MS, Finak G, Evans AJ, Metcalf JL, Hast BE, Hanna SC, Wondergem B, Furge KA, Irwin MS, Kim WY, Teh BT, Grinstein S, Park M, Marsden PA, Ohh M,

Journal:Nat Med

PubMed ID:19252501

'Tumor hypoxia is associated with disease progression, resistance to conventional cancer therapies and poor prognosis. Hypoxia, by largely unknown mechanisms, leads to deregulated accumulation of and signaling via receptor tyrosine kinases (RTKs) that are critical for driving oncogenesis. Here, we show that hypoxia or loss of von Hippel-Lindau protein--the principal ... More

Quantitative measurement of mast cell degranulation using a novel flow cytometric annexin-V binding assay.

Authors:Demo SD, Masuda E, Rossi AB, Throndset BT, Gerard AL, Chan EH, Armstrong RJ, Fox BP, Lorens JB, Payan DG, Scheller RH, Fisher JM

Journal:Cytometry

PubMed ID:10404150

'BACKGROUND: Mast cells are primary mediators of allergic inflammation. Antigen-mediated crosslinking of their cell surface immunoglobulin E (IgE) receptors results in degranulation and the release of proinflammatory mediators including histamine, tumor necrosis factor-alpha, and leukotrienes. METHODS: Mast cells were stimulated to degranulate by using either IgE crosslinking or ionophore treatment. ... More

Inhibition of the vacuolar H(+)-pump with bafilomycin A1 does not induce acrosome reaction or activate proacrosin in mouse spermatozoa.

Authors:Codelia VA, Cortes CJ, Moreno RD

Journal:Biochem Biophys Res Commun

PubMed ID:16236270

'Acrosomal protease activation is regarded as an important event triggered by acrosomal reaction and leading to sperm passage through zona pellucida. Mammalian acrosome has an internal acid pH that probably helps to maintain inactive proenzymes that otherwise could be precociously activated and prevent normal fertilization. In this work, we have ... More

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