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Citas y referencias (5)
Invitrogen™
Streptavidin, β-galactosidase conjugate
El conjugado de estreptavidina ß-galactosidasa puede usarse para detectar biotina en un esquema de amplificación de señal junto con sustratosMás información
| Número de catálogo | Cantidad |
|---|---|
| S931 también denominado S-931 | 1 mg |
Número de catálogo S931
también denominado S-931
Precio (EUR)
598,00
Each
Cantidad:
1 mg
Precio (EUR)
598,00
Each
El conjugado de estreptavidina ß-galactosidasa puede usarse para detectar biotina en un esquema de amplificación de señal junto con sustratos cromogénicos o fluorogénicos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de productoConjugado de estreptavidina (fluorescente)
Cantidad1 mg
Condiciones de envíoHielo húmedo
Conjugadobeta-galactosidasa
FormularioSólido
Unit SizeEach
Contenido y almacenamiento
Almacenar en congelador (de -5 a -30 °C).
Citations & References (5)
Citations & References
Abstract
Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.
Journal:J Immunol Methods
PubMed ID:2558138
'A novel signal amplification method, catalyzed reporter deposition (CARD), and its application to immunoassays is described. The method involves utilizing an analyte-dependent reporter enzyme (ADRE) to catalyze the deposition of additional reporter on the surface in a solid-phase immunoassay. In the examples described, deposition of reporter is facilitated by using
Fluorescence probe studies of the state of tropomyosin in reconstituted muscle thin filaments.
Journal:Biochemistry
PubMed ID:3663633
'The monomer fluorescence of N-(1-pyrenyl)maleimide-labeled tropomyosin bound to F-actin (PTm-actin) increases when myosin subfragment 1 (S1) binds to actin and is half complete when only approximately 1 S1 is bound to 7 actin subunits [Ishii, Y., & Lehrer, S. S. (1985) Biochemistry 24, 6631-6638]. Similar studies of the binding of
Fluorescence sandwich enzyme-linked immunosorbent assay for detecting human interleukin-2 receptors.
Journal:J Immunol Methods
PubMed ID:2453583
A very sensitive fluorescence sandwich enzyme-linked immunosorbent assay (FS-ELISA) for the detection of soluble and cell-associated human interleukin-2 receptors (IL-2R) has been developed. For use in this assay system, two anti-human IL-2R monoclonal antibodies, H 48 and biotinylated HA 26, were selected from six monoclonal antibodies directed against different epitopes
Measurement of antigen-specific mouse IgE by a fluorometric reverse (IgE-capture) ELISA.
Journal:J Immunol Methods
PubMed ID:2783445
A reverse, or IgE-capture, enzyme-linked immunosorbent assay (ELISA) for measuring ovalbumin-specific IgE antibody in the serum of immunized mice has been developed. Microplate wells were first coated with a commercial anti-mouse IgE rat monoclonal antibody, and then incubated with two-fold serial dilutions of test sera with 10% normal mouse serum
TNF-alpha pretreatment prevents subsequent activation of cultured brain cells with TNF-alpha and hypoxia via ceramide.
Journal:Am J Physiol
PubMed ID:10329967
We have developed a cellular model in which cultured astrocytes and brain capillary endothelial cells preconditioned with tumor necrosis factor-alpha (TNF-alpha) fail to upregulate intercellular adhesion molecule-1 (ICAM-1) protein (80% inhibition) and mRNA (30% inhibition) when challenged with TNF-alpha or exposed to hypoxia. Inasmuch as ceramide is known to mediate