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Citations & References (6)
PFASTBAC 1 EXPRESSION VECTOR
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PFASTBAC 1 EXPRESSION VECTOR
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Citations & References (6)
Citations & References
Abstract
Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells.
Journal:Stem Cells
PubMed ID:17420229
'Human embryonic stem (hES) cells as a renewable cell source have great prospective applications in both developmental biology research and regenerative medicine. To realize these potentials, the development of effective and safe genetic manipulation methods in hES cells is an obvious demand. We report here that baculoviral vectors were able
Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase.
Journal:Proc Natl Acad Sci U S A
PubMed ID:10618442
A member of the phosphodiesterase (PDE)7 family with high affinity and specificity for cAMP has been identified. Based on sequence homologies, we designate this PDE as PDE7B. The full-length cDNA of PDE7B is 2399 bp, and its ORF sequence predicts a protein of 446 amino acids with a molecular mass
The orexin OX1 receptor regulates Ca2+ entry via diacylglycerol-activated channels in differentiated neuroblastoma cells.
Journal:J Neurosci
PubMed ID:17050705
We studied the cellular response to orexin type 1 receptor (OX1R) stimulation in differentiated IMR-32 neuroblastoma cells. In vitro differentiation of IMR-32 cells with 5-bromo-2'-deoxyuridine leads to a neuronal phenotype with long neurite extensions and an upregulation of mainly N-type voltage-gated calcium channels. Transduction of differentiated IMR-32 cells with baculovirus
RAD51C Interacts with RAD51B and Is Central to a Larger Protein Complex in Vivo Exclusive of RAD51.
Journal:J Biol Chem
PubMed ID:11744692
RAD51B and RAD51C are two of five known paralogs of the human RAD51 protein that are thought to function in both homologous recombination and DNA double-strand break repair. This work describes the in vitro and in vivo identification of the RAD51B/RAD51C heterocomplex. The RAD51B/RAD51C heterocomplex was isolated and purified by
Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids.
Journal:Proc Natl Acad Sci U S A
PubMed ID:11805319
Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana,