Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Catalog Number | Unit Size | Price (EUR) | Availability | Quantity | |
---|---|---|---|---|---|
11330032 | 500 mL | Price: 36,75 Your price: | - | ||
31330038 also known as 31330-038 | 500 mL | Price: 28,27 Online price: 12,65 Your price: | - | ||
31330095 also known as 31330-095 | 10 x 500 mL | Price: 255,00 Your price: | - | ||
11330057 | 10 x 500 mL | Price: 247,00 Online price: 187,65 Your price: | - | ||
11330107 | 10 L | Price: 429,00 Your price: | - |
Dulbecco's Modified Eagle Medium:Nutrient Mixture F-12 (DMEM / F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM / F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of Gibco™ DMEM / F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
With |
• L-glutamine |
• HEPES |
• Phenol Red |
The complete formulation is available.Gibco™ DMEM / F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components.
DMEM / F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM / F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM / F-12 uses a sodium bicarbonate buffer system and therefore requires a 5-10% CO2 environment to maintain physiological pH.