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Citations & References (6)
Gibco™
HEPES (1M)
Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. It is membrane impermeable, relatively stable, and has limited interference in key processes.
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| Catalog Number | Quantity |
|---|---|
| 15630080 | 100 mL |
| 15630106 | 20 mL |
Catalog number 15630080
Price (EUR)
141,00
Each
Quantity:
100 mL
Price (EUR)
141,00
Each
Gibco HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator.
Gibco HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a good buffering choice for many cell culture systems because it:
- Is membrane impermeable
- Has limited effect on biochemical reactions
- Is chemically and enzymatically stable
- Has a very low visible and UV light absorbance
cGMP manufacturing and quality system
For supply chain continuity, we manufacture this product at two separate facilities located in Grand Island, NY, and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Chemical Name or MaterialZwitterionic Organic Chemical Buffer
ColorClear
FormulationN-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid
Recommended StorageStorage conditions: 2°C to 8°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
SterilitySterile
Sterilization MethodSterile-filtered
For Use With (Application)Chromatin Biology
GradeBiochemical
Physical FormLiquid
Quantity100 mL
Solution TypeBuffer
Unit SizeEach
Citations & References (6)
Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Journal:Experimental cell research
PubMed ID:38499143
Preparation and characterization of toxic Abeta aggregates for structural and functional studies in Alzheimer's disease research.
Journal:Nat Protoc
PubMed ID:20539293
'The amyloid cascade hypothesis, supported by strong evidence from genetics, pathology and studies using animal models, implicates amyloid-beta (Abeta) oligomerization and fibrillogenesis as central causative events in the pathogenesis of Alzheimer''s disease (AD). Today, significant efforts in academia, biotechnology and the pharmaceutical industry are devoted to identifying the mechanisms by
Investigating conversion of mechanical force into biochemical signaling in three-dimensional chondrocyte cultures.
Journal:Nat Protoc
PubMed ID:19478808
The culture of chondrocytes embedded within agarose hydrogels maintains chondrocytic phenotype over extended periods and allows analysis of the chondrocyte response to mechanical forces. The mechanisms involved in the transduction of a mechanical stimulus to a physiological process are not completely deciphered. We present protocols to prepare and characterize constructs
Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.
Journal:Neuron
PubMed ID:11779481
Many N-methyl-D-aspartate receptor (NMDAR) channel blockers that have therapeutic potential can be trapped in the closed state. Using a combination of the substituted cysteine accessibility method and open channel blockers, we found that the M3 segment forms the core of the extracellular vestibule, including a deep site for trapping blockers.
Polycystin-1, the product of the polycystic kidney disease 1 gene, co-localizes with desmosomes in MDCK cells.
Journal:Hum Mol Genet
PubMed ID:11063733
Polycystin-1 is a novel protein predicted to be a large membrane-spanning glycoprotein with an extracellular N-terminus and an intracellular C-terminus, harboring several structural motifs. To study the subcellular localization, antibodies raised against various domains of polycystin-1 and against specific adhesion complex proteins were used for two-color immunofluorescence staining. In Madine