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Citations & References (5)
Thermo Scientific™
SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)
Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactiveRead more
| Catalog Number | Quantity |
|---|---|
| 22329 | 50 mg |
Catalog number 22329
Price (EUR)
318,65
Online Exclusive
424,00Save 105,35 (25%)
Each
Quantity:
50 mg
Price (EUR)
318,65
Online Exclusive
424,00Save 105,35 (25%)
Each
Thermo Scientific Pierce SIAB is a mid-length (10.6 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
Features of SIAB:
• Reactive groups: NHS ester and iodoacetyl
• Reactive toward: amino and sulfhydryl groups
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-SIAB
• Used to prepare stable enzyme-IgG conjugates
• Forms conjugates with liposomes
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)
Features of SIAB:
• Reactive groups: NHS ester and iodoacetyl
• Reactive toward: amino and sulfhydryl groups
• Non-cleavable
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-SIAB
• Used to prepare stable enzyme-IgG conjugates
• Forms conjugates with liposomes
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
Related Products
Sulfo-SIAB (sulfosuccinimidyl (4-iodoacetyl)aminobenzoate)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell PermeabilityYes
DescriptionSIAB
FormPowder
Labeling MethodChemical Labeling
Molecular Weight (g/mol)402.14
PEGylatedNo
Product LinePierce
Quantity50 mg
Reactive MoietyHaloacetyl, NHS Ester
Shipping ConditionAmbient
SolubilityDMF, DMSO
Spacer Arm Length10.6 Å
Water SolubleNo
Chemical ReactivityAmine-Sulfhydryl
CleavableNo
Crosslinker TypeHeterobifunctional
FormatStandard
Product TypeCrosslinker
SpacerMedium (10 to 30 Å)
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C protected from light.
Frequently asked questions (FAQs)
Can you provide the shelf-life for SIAB (succinimidyl (4-iodoacetyl)aminobenzoate)?
Citations & References (5)
Citations & References
Abstract
Chip-based genotyping by mass spectrometry.
Journal:Proc Natl Acad Sci U S A
PubMed ID:10468554
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip
Covalent attachment of synthetic DNA to self-assembled monolayer films.
Journal:Nucleic Acids Res
PubMed ID:8760890
'The covalent attachment of thiol-modified DNA oligomers; to self-assembled monolayer silane films on fused silica and oxidized silicon substrates is described. A heterobifunctional crosslinking molecule bearing both thiol- and amino-reactive moieties was used to tether a DNA oligomer (modified at its terminus with a thiol group) to an aminosilane film
Gbeta subunit interacts with a peptide encoding region 956-982 of adenylyl cyclase 2. Cross-linking of the peptide to free Gbetagamma but not the heterotrimer.
Journal:J Biol Chem
PubMed ID:8900107
The region encoded by amino acids 956-982 of adenylyl cyclase 2 is important for Gbetagamma stimulation. Interactions of a peptide encoding the 956-982 region of adenylyl cyclase 2 (QEHAQEPERQYMHIGTMVEFAYALVGK (QEHA peptide)) with Gbetagamma subunits were studied. QEHA peptide was covalently attached to beta subunit of free Gbetagamma by the cross-linker
Mitochondrial protein import: recognition of internal import signals of BCS1 by the TOM complex.
Journal:Mol Cell Biol
PubMed ID:12640110
BCS1, a component of the inner membrane of mitochondria, belongs to the group of proteins with internal, noncleavable import signals. Import and intramitochondrial sorting of BCS1 are encoded in the N-terminal 126 amino acid residues. Three sequence elements were identified in this region, namely, the transmembrane domain (amino acid residues
Use of immobilized PCR primers to generate covalently immobilized DNAs for in vitro transcription/translation reactions.
Journal:Nucleic Acids Res
PubMed ID:10606673
We have developed a novel biochemical method to simultaneously amplify and immobilize a target gene onto insoluble particles using PCR. This method employs the covalent attachment of one of two PCR primers to a particle surface either directly during DNA synthesis of the primer or post-DNA synthesis, through the use