CaptureSelect™ FcXP Ligand Leakage ELISA

CaptureSelect FcXP Ligand Leakage ELISA is designed to detect possible leached ligand of antibodies or antibody fragments purified using CaptureSelect FcXP Affinity Matrix. The FcXP affinity ligand is a 13-kD recombinant protein, expressed in yeast and covalently coupled to a chromatography matrix for high chemical stability and low leakage. During the chromatography process small amounts of the affinity ligand can leach off of the chromatography support and co-elute with the protein.

This ELISA provides typical sensitivity and accurate quantitation of possible leached ligand greater than or equal to 1 ng/mL. The CaptureSelect FcXP Ligand Leakage ELISA can be used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.