Two standard options are available:
- As a set of linear DNA fragments, ready for restriction enzyme digestion and subcloning.
- Subcloned into any vector and delivered as a glycerol stock and plasmid preparation.
Is it possible to introduce diversity with nucleotide mixes other than the fixed ratios defined by ambiguous nucleotides for your GeneArt Libraries?
Yes, GeneArt Combinatorial Libraries can be randomized using any nucleotide mix you require, down to single digit percentages of given nucleotides (“dirty bottle” approach).
Where can I find the IUPAC nucleotide ambiguity codes?
We recommend this site: http://www.chick.manchester.ac.uk/SiteSeer/IUPAC_codes.htmL
