Human Epidermal Keratinocytes, Pooled (HEKp)
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Gibco™

Human Epidermal Keratinocytes, Pooled (HEKp)

Human Epidermal Keratinocytes, pooled (HEKp) from Invitrogen are normal cells isolated from multiple neonatal foreskins. These cells are optimal forRead more
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Catalog NumberQuantity
A134011 vial
Catalog number A13401
Price (EUR)
726,00
Each
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Quantity:
1 vial
Price (EUR)
726,00
Each
Add to cart
Human Epidermal Keratinocytes, pooled (HEKp) from Invitrogen are normal cells isolated from multiple neonatal foreskins. These cells are optimal for investigating the molecular and biochemical bases of normal and disease processes of the skin. They have been cryopreserved at the end of the primary culture stage in animal origin-free media containing 10% DMSO. Use HEKp for your research into fibrosis, wound healing, irritancy, and diseases such as psoriasis.
Guaranteed to have ≥70% viability upon thawing
• Each vial guaranteed to reach ≥15 population doublings after thaw
• Sufficient cells to seed ≥100 cm2
• Tested for potential biological contaminants
• Optimized for use in EpiLife™ serum-free media

Get More Physiologically Relevant Results with Invitrogen Primary Cells

Human primary cell cultures closely mimic the in vivo state and thus generate more physiologically relevant data. Our Human Epidermal Keratinocytes are isolated and pooled from 4-6 neonatal foreskins. This process is robust and yields large donor lots to ensure availability of consistent product for your studies.

Reliable Performance and Purity
Primary cells from Invitrogen are guaranteed to have ≥70% viability upon thawing with each vial containing sufficient cells to seed ≥100 cm2 of surface area. Performance testing guarantees each vial of HEKp will reach ≥15 population doublings after thaw. In addition, each lot is tested for potential biological contaminants--HIV-1, Hepatitis B, Hepatitis C viruses, mycoplasma, bacteria, yeast, and other fungi-- to ensure quality and robustness.

Optimized for Use with Gibco™ Media
Human Epidermal Keratinocytes, pooled, from Invitrogen have been optimized for use with our serum-free media, specifically EpiLife™ Medium with 60 μM calcium and Human Keratinocyte Growth Supplement (HKGS). Preparation of supplemented media is a simple process; the final solution can be stored for up to 30 days at 4°C.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Learn more about our complete system of media and supplements for culturing Human Epidermal Keratinocytes
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell Passage NumberPrimary culture stage
ClassificationSerum-Free
Donor AttributesNormal
Donor SourcePooled
FormatVial
No. of Cells≥1 x 106
Product LineGibco
Product TypeHuman Epidermal Keratinocyte
Quantity1 vial
Tested ForHepatitis B (HBV), Hepatitis C (HCV), Sterility Testing (Bacteria & Fungi), Mycoplasma, Growth Performance Assay, HIV-1
AgeNewborn (14 days or less)
Cell TypeKeratinocyte Cells
Culture TypeAdherent Cell Culture
FormCryopreserved
GenderMale
SpeciesHuman
Unit SizeEach
Contents & Storage
≥ 1 × 106 viable cells

Frequently asked questions (FAQs)

I see large, flat pancake-looking cells in my keratinocyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care an attention, the culture should yield at least 25 population doublings.

I see large, flat pancake-looking cells in my Human Keratinodyte culture. What are they?

These are senescent cells, and this is normal for adult keratinocyte culture. You always have a population of cells that are old and no longer proliferate. However younger cells, which are small in size, should keep proliferating. When a culture gets older, you see more and more large cells, and the culture will eventually stop growing. With the right care and attention, the culture should yield at least 25 population doublings.

What is the doubling time for Human Keratinodyte?

Each lot is tested for doubling time and the results are listed on the COA.

Can I specify a donor type for the primary cells you offer?

This information also appears on the COA.

What is the difference between basal cells or basal keratinocytes?

These are all the same. Sometimes Keratinocytes are referred to as basal cells or basal keratinocytes.