Search
Citations & References (15)
Invitrogen™
Phalloidin Labeling Probes
Achieve precise and reliable F-actin staining with fluorescent and biotinylated phalloidins. Phalloidin conjugates are widely used in imaging applications to selectively label F-actin in a variety of sample types including fixed and permeabilized cells, tissue sections, and cell-free experiments.
Have Questions?
Change view
| Catalog Number | Color | Excitation Wavelength Range | Dye Type |
|---|---|---|---|
| A22285 | Near-infrared | 663/690 nm | Alexa Fluor™ 660 |
| A22281 | Blue | 346/442 nm | Alexa Fluor™ 350 |
| A30104 | Violet | 405/450 nm | Alexa Fluor™ Plus 405 |
| A12379 | Green | 495/518 nm | Alexa Fluor™ 488 |
| O7466 | Green | 496/520 nm | Oregon Green™ 488 |
| F432 | Green | 496/516 nm | FITC (Fluorescein) |
| A22282 | Yellow | 531/554 nm | Alexa Fluor™ 532 |
| R415 | Red-orange | 540/565 nm | TRITC |
| A22283 | Orange | 556/570 nm | Alexa Fluor™ 546 |
| A34055 | Orange | 555/565 nm | Alexa Fluor™ 555 |
| A30106 | Orange | 555/565 nm | Alexa Fluor Plus 555 |
| B3475 | Red | 558/569 nm | BODIPY™ |
| A12380 | Orange-red | 578/600 nm | Alexa Fluor™ 568 |
| A12381 | Red | 581/609 nm | Alexa Fluor™ 594 |
| T7471 | Red | 591/608 nm | Texas Red™ |
| A22284 | Far-red | 632/647 nm | Alexa Fluor™ 633 |
| A34054 | Far-red | 633/647 nm | Alexa Fluor™ 635 |
| A22287 | Far-red | 650/668 nm | Alexa Fluor™ 647 |
| A30107 | Far-red | 650/668 nm | Alexa Fluor Plus 647 |
| A22286 | Near-infrared | 679/702 nm | Alexa Fluor™ 680 |
| A30105 | Near-infrared | 758/784 nm | Alexa Fluor™ Plus 750 |
| B7474 | None | None | Biotin-XX |
| P3457 | None | None | Phalloidin (unlabeled) |
Catalog number A22285
Price (EUR)
860,65
Online Exclusive
916,00Save 55,35 (6%)
Each
Color:
Near-infrared
Excitation Wavelength Range:
663/690 nm
Dye Type:
Alexa Fluor™ 660
Price (EUR)
860,65
Online Exclusive
916,00Save 55,35 (6%)
Each
Fluorescent and biotinylated phalloidins are water soluble and bind to filamentous actin (F-actin) with nanomolar affinity, making them convenient probes for labeling, identifying, and quantifying F-actin in cryopreserved tissue sections, fixed and permeabilized cells, and cell-free experiments. Phalloidin conjugates bind similarly to actin from various species, including plants and animals, enabling staining of the cytoskeleton in a wide range of samples.
A variety of phalloidin conjugates for filamentous (F-actin) staining are available, including fluorescent Alexa Fluor and Alexa Fluor Plus phalloidins, along with phalloidins conjugated to classic fluorescent dyes such as BODIPY, fluorescein, and rhodamine. Phalloidin staining is spectrally compatible with other fluorescent stains used in cellular analyses such as GFP/RFP, Qdot nanocrystals, and other Alexa Fluor conjugates and antibodies. Biotin‐XX Phalloidin can be used to visualize actin filaments via fluorescent streptavidin tags or standard enzyme-mediated avidin/streptavidin techniques such as in electron microscopy. Unlabeled phalloidin is available for use as a control in blocking F‐actin staining or in promoting polymerization.
Phalloidin conjugates bind to both large and small actin filaments with similar affinity in a 1:1 stoichiometry between phallotoxin and actin subunits. They do not bind G-actin monomers.
Alexa Fluor and Alexa Fluor Plus phalloidin conjugates for F-actin staining
Fluorescent Alexa Fluor dye conjugates of phalloidin are popular F-actin stains, offering color choices across the full spectral range. These phalloidin conjugates provide researchers with fluorescent probes that are superior in brightness and photostability compared to other spectrally similar conjugates.
Alexa Fluor Plus Phalloidin conjugates retain the same specificity for actin but offer 3-5 times greater sensitivity and brightness compared to the corresponding Alexa Fluor Phalloidin conjugate. This increased brightness is beneficial for challenging F-actin imaging, such as the super‐resolution microscopy methods SIM and STORM, and for reliable staining of actin stress fibers.
Features of phalloidin probes
- High specificity—binds selectively to F-actin, which allows for precise labeling of actin filaments in fixed cells and cryopreserved tissues
- Strong affinity—nanomolar binding affinity for F-actin, which ensures stable and reliable actin staining
- Extensive fluorescent conjugate options—over twenty conjugated varieties of phalloidin
- Compatibility with fixed samples—typically used with fixed cells and tissues, making them suitable for actin staining in detailed structural studies, immunofluorescence staining, and IHC applications
- Multiplexing capability—the wide availability of phalloidin conjugates enables their use in combination with other fluorescent probes and antibodies for multiplex imaging. Biotinylated phalloidin can be made use of in downstream streptavidin steps.
- Quantitative analysis—can be used for quantitative analysis of F-actin distribution and density within cells, aiding in the study of cytoskeletal dynamics. The unlabeled phalloidin can be titrated as a control.
- Ease of use—staining is straightforward and quick
- Excellent stability—exhibit good photostability, which is essential for prolonged imaging sessions and time-lapse studies
- Wide applicability—used for a range of applications, including studying cell morphology, motility, and the effects of drugs on the actin cytoskeleton
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorNear-infrared
Dye TypeAlexa Fluor™ 660
Excitation Wavelength Range663/690 nm
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Confocal Microscope, Compatible Cy5/Cy5.5 filter set
Product LineAlexa Fluor
Quantity300 Units
Shipping ConditionRoom Temperature
Label TypeAlexa Fluor Dyes
Product TypePhalloidin
SubCellular LocalizationActin, Cytoskeleton
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Frequently asked questions (FAQs)
Can I combine Click-iT or Click-iT Plus reactions with phalloidin conjugates used for actin staining?
I'm trying to label my paraffin sections for F-actin with a phalloidin conjugate, but I'm not seeing any signal. Why?
Citations & References (15)
Citations & References
Abstract
Myosin Va maneuvers through actin intersections and diffuses along microtubules.
Journal:Proc Natl Acad Sci U S A
PubMed ID:17360524
'Certain types of intracellular organelle transport to the cell periphery are thought to involve long-range movement on microtubules by kinesin with subsequent handoff to vertebrate myosin Va (myoVa) for local delivery on actin tracks. This process may involve direct interactions between these two processive motors. Here we demonstrate using single
Sidekicks: synaptic adhesion molecules that promote lamina-specific connectivity in the retina.
Journal:Cell
PubMed ID:12230981
A major determinant of specific connectivity in the central nervous system is that synapses made by distinct afferent populations are restricted to particular laminae in their target area. We identify Sidekick (Sdk)-1 and -2, homologous transmembrane immunoglobulin superfamily molecules that mediate homophilic adhesion in vitro and direct laminar targeting of
Two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis.
Journal:Plant Cell Physiol
PubMed ID:15047875
We show that two distinct distributions of F-actin are present in the hyphal apex of the oomycete Achlya bisexualis, that have been chemically fixed with a combination of methylglyoxal and formaldehyde and stained with Alexa phalloidin. In approximately one half of the hyphae examined, an F-actin depleted zone within the
A role for PKC-epsilon in Fc gammaR-mediated phagocytosis by RAW 264.7 cells.
Journal:J Cell Biol
PubMed ID:12499353
Protein kinase C (PKC) plays a prominent role in immune signaling, and the paradigms for isoform selective signaling are beginning to be elucidated. Real-time microscopy was combined with molecular and biochemical approaches to demonstrate a role for PKC- epsilon in Fc gamma receptor (Fc gammaR)-dependent phagocytosis. RAW 264.7 macrophages were
The HIV-1 pathogenicity factor Nef interferes with maturation of stimulatory T-lymphocyte contacts by modulation of N-Wasp activity.
Journal:J Biol Chem
PubMed ID:16687395
The Nef protein is a key determinant of human immunodeficiency virus (HIV) pathogenicity that, among other activities, sensitizes T-lymphocytes for optimal virus production. The initial events by which Nef modulates the T-cell receptor (TCR) cascade are poorly understood. TCR engagement triggers actin rearrangements that control receptor clustering for signal initiation