Ion SingleSeq™ 96 Kit
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Ion Torrent™

Ion SingleSeq™ 96 Kit

The Ion SingleSeq 96 Kit is designed for low cell number input library construction prior to low-pass genome sequencing detectionRead more
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Catalog NumberQuantity
A3476396 reactions
Catalog number A34763
Price (EUR)
13 520,00
Each
Add to cart
Quantity:
96 reactions
Price (EUR)
13 520,00
Each
Add to cart
The Ion SingleSeq 96 Kit is designed for low cell number input library construction prior to low-pass genome sequencing detection of copy number events. Requiring as little as 6 pg of DNA from a single cell, or input from multiple cells, this kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples.

The Ion SingleSeq 96 Kit is intended to be used with the Ion ReproSeq PGS Kit for the Ion S5 System for the detection of chromosomal aneuploidies and chromosome arm copy number events (>48 Mb). The Ion ReproSeq PGS Kit is used with the Ion Chef System and the Ion S5 or Ion S5 XL sequencing system to enable automated template preparation, chip loading, and sequencing with disposable reagent cartridges for fast and simple instrument set-up to minimize hands-on time. Compatible kits include:
• Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run) (Cat. No. A34900)
• Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run) (Cat. No. A34901)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)Ion GeneStudio S5 System, Ion PGM™ System, Ion Chef™ System
LibrariesFragment Library
Product Type96 Kit
Quantity96 reactions
Sequencing TypeGenome & DNA Sequencing
Shipping ConditionApproved for shipment on Wet or Dry Ice
Throughput96 Samples
Workflow StepLibrary Generation
Product LineReproSeq
Starting MaterialDNA
Unit SizeEach
Contents & Storage
Store at –10 to –30°C.

Frequently asked questions (FAQs)

Does the Ion SingleSeq 96 Kit include reagents for template preparation, sequencing, and chips?

No, the Ion SingleSeq 96 Kit only includes reagents to prepare the library. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

What is included in the Ion SingleSeq 96 Kit?

The Ion SingleSeq 96 Kit includes reagents to extract, amplify, and barcode genomic DNA for preparation of libraries from up to 96 samples. For a detailed list, please go here (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0016712_IonReproSeqPGS_S5_UG.pdf).

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Where can I find the sequences for the IonCode barcodes?

The sequences for the barcodes can be found in the Barcodes section of your Torrent Browser. You can also download a .csv file containing the full list of sequences in that set.

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.

Are the Ion ReproSeq PGS Kit subkits available separately?

Yes, the Ion SingleSeq Kit (Cat. No. A28955, 24 reactions/kit), or Ion SingleSeq 96 Kit (Cat. No. A34763, 96 reactions/kit) are available separately.

The Ion ReproSeq PGS Kits - Ion S5 System, are also available separately and include reagents to prepare templates and load 4 chips on the Ion Chef Instrument, and sequence the chips on the Ion S5 or Ion S5 XL Sequencer.
- Ion ReproSeq PGS Kit with Ion 520 Chips (24 samples/run), Cat. No. A34900
- Ion ReproSeq PGS Kit with Ion 530 Chips (96 samples/run), Cat. No. A34901

Find additional tips, troubleshooting help, and resources within our Next-Generation Sequencing Support Center.