Guanidine Thiocyanate
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Invitrogen™

Guanidine Thiocyanate

Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and isRead more
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Catalog NumberQuantity
AM9422500 g
Catalog number AM9422
Price (EUR)
478,00
Each
Add to cart
Quantity:
500 g
Price (EUR)
478,00
Each
Add to cart
Guanidine Thiocyanate is an ultrapure, molecular biology grade reagent. It is free of detectable nuclease and protease activity and is a strong protein denaturant, as both the guanidinium cation and the thiocyanate anion are chaotropic agents. It is provided in one bottle containing 500 g. It is recommended for isolation of RNA, especially for tissues such as pancreas with high levels of RNase activity. In solution, it is known as guanidinium thiocyanate. Guanidine Thiocyanate is thoroughly tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Specifications:
Appearance: White crystals Molecular Weight: 118.16 Purity:
>99% Stability: Unlimited under dry ambient conditions. Keep in dark container.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)RNA Extraction
Product LineAmbion
Product TypeGuanidine Isothiocyanate
Purity>99%
Quantity500 g
Shipping ConditionRoom Temperature
FormPowder
Unit SizeEach
Contents & Storage
Store at room temperature.

Frequently asked questions (FAQs)

What salts can be used to precipitate total RNA? What are the advantages of each? Do they require ethanol?

The three salts that can be used are:

- Guanidine thiocyanate, which requires ethanol. Guanidine thiocyanate is a common agent used for isolating RNA and we recommend it especially for tissues high in ribonuclease activity, such as the pancreas or spleen.
- Ammonium acetate, which requires ethanol. Ammonium acetate is useful when reducing coprecipitation of unwanted dNTPs and oligosaccharides. However, it should not be used when the nucleic acid will be phosphorylated using T4 polynucleotide kinase, since this enzyme is inhibited by ammonium ions.
- Lithium chloride, which does not require ethanol. LiCl is very effective in precipitating RNA but does not efficiently precipitate protein or DNA. It also does not precipitate unincorporated nucleotides.