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Thermo Scientific Chemicals
4-Methylumbelliferyl-beta-D-glucuronide, 98%
CAS: 6160-80-1 | C16H16O9 | 352.295 g/mol
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Catalog number B21190.MF
also known as B21190-MF
Price (EUR)
51,80
Each
Quantity:
50 mg
Price (EUR)
51,80
Each
Chemical Identifiers
CAS6160-80-1
IUPAC Name(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-[(4-methyl-2-oxo-2H-chromen-7-yl)oxy]oxane-2-carboxylic acid
Molecular FormulaC16H16O9
InChI KeyARQXEQLMMNGFDU-JHZZJYKESA-N
SMILESCC1=CC(=O)OC2=CC(O[C@@H]3O[C@@H]([C@@H](O)[C@H](O)[C@H]3O)C(O)=O)=CC=C12
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Specifications
Specification Sheet
Specification SheetIdentification (FTIR)Conforms
Assay (HPLC)≥97.5%
CommentOptical Rotation varies with water content
Water Content (Karl Fischer Titration)≤20%
Optical Rotation-99° to -120° (c=0.1 in water)
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It is a fluorogenic substrate for measuring of β-glucoronidiase activity, for rapid sensitive identification of Escherichia coli. MUG is a substrate for glucuronidase. It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
Applications
It is a fluorogenic substrate for measuring of ß-glucoronidiase activity, for rapid sensitive identification of Escherichia coli. MUG is a substrate for glucuronidase. It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.
Solubility
Soluble in hot water.
Notes
Light sensitive. Store away from alkali metals bases and light. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
It is a fluorogenic substrate for measuring of ß-glucoronidiase activity, for rapid sensitive identification of Escherichia coli. MUG is a substrate for glucuronidase. It is ideal for plant molecular study as a way to examine gene expression under various promoters. And while it works well for measuring activity in plant lysate and leaf disks, it can also work in whole plant assays.
Solubility
Soluble in hot water.
Notes
Light sensitive. Store away from alkali metals bases and light. Keep the container tightly closed and place it in a cool, dry and well ventilated condition. Store in freezer at -20°C.
RUO – Research Use Only
General References:
- Farnleitner AH, et al. Hydrolysis of 4-methylumbelliferyl-beta-D-glucuronide in differing sample fractions of river waters and its implication for the detection of fecal pollution.Water Res.200236(4), 975-81.
- Ekholm DF and Hirshfield IN. Rapid methods to enumerate Escherichia coli in foods using 4-methylumbelliferyl-beta-D-glucuronide.J AOAC Int.200184(2), 407-15.