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Citations & References (173)
Invitrogen™
Brefeldin A (from Penicillium brefeldianum)
The fungal metabolite brefeldin A (BFA) has multiple targets in cells and has proven valuable for dissecting the cellular processes,Read more
| Catalog Number | Quantity |
|---|---|
| B7450 | 5 mg |
Catalog number B7450
Price (EUR)
222,00
Each
Quantity:
5 mg
Price (EUR)
222,00
Each
The fungal metabolite brefeldin A (BFA) has multiple targets in cells and has proven valuable for dissecting the cellular processes, including vesicle formation and kinesin distribution, involved in exporting newly synthesized proteins. We are pleased to offer the natural brefeldin A isolated from Penicillium brefeldianum. Exposing cells to BFA causes a distortion in intracellular protein traffic from the ER to the Golgi apparatus and the eventual loss of Golgi apparatus morphology; removal of BFA completely reverses these effects. BFA also alters the morphology of endosomes and lysosomes.
Dissolve in high-quality anhydrous DMSO or ethanol to prepare a stock concentration up to 1 mM.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Label TypeUnlabeled
Molecular FormulaC16H24O4
Molecular Weight (g/mol)280.36
Quantity5 mg
Recommended StorageStore in freezer (-5°C to -30°C)
Shipping ConditionRoom Temperature
Physical FormPowder
Product TypeSphingolipid
Unit SizeEach
Citations & References (173)
Citations & References
Abstract
Nocodazole-dependent transport, and brefeldin A--sensitive processing and sorting, of newly synthesized membrane proteins in cultured neurons.
Journal:J Neurosci
PubMed ID:7790910
The envelope glycoproteins of Semliki Forest virus (SFV), Vesicular Stomatitis virus (VSV), and Influenza Fowl Plague virus (FPV) are vectorially targeted in neurons to the plasma membrane of dendrites (SFV and VSV) and axons (FPV). To gain insight into the mechanisms responsible for such polarized delivery we have examined the
Mammalian Cdc42 is a brefeldin A-sensitive component of the Golgi apparatus.
Journal:J Biol Chem
PubMed ID:8900167
'In this study, we have used immunocytochemical and fractionation approaches to provide a description of the localization of the mammalian Cdc42 protein (designated Cdc42Hs) in vivo. A specific anti-peptide antibody was generated against the C-terminal region of Cdc42Hs. Using affinity-purified preparations of this antibody in indirect immunofluorescence experiments, Cdc42Hs was
Molecular cloning of a novel 97-kd Golgi complex autoantigen associated with Sjögren's syndrome.
Journal:Arthritis Rheum
PubMed ID:9324025
'OBJECTIVE: To identify a Golgi complex autoantigen bound by Sjögren''s syndrome (SS) autoantibodies. METHODS: Serum from a patient with secondary SS and anti-Golgi antibodies was used as a probe to isolate a complementary DNA (cDNA) insert from a HeLa cDNA library. RESULTS: A 3.7-kb cDNA encoding a 56-kd recombinant protein
An ordered inheritance strategy for the Golgi apparatus: visualization of mitotic disassembly reveals a role for the mitotic spindle.
Journal:J Cell Biol
PubMed ID:9585414
'During mitosis, the ribbon of the Golgi apparatus is transformed into dispersed tubulo-vesicular membranes, proposed to facilitate stochastic inheritance of this low copy number organelle at cytokinesis. Here, we have analyzed the mitotic disassembly of the Golgi apparatus in living cells and provide evidence that inheritance is accomplished through an
Direct pathway from early/recycling endosomes to the Golgi apparatus revealed through the study of shiga toxin B-fragment transport.
Journal:J Cell Biol
PubMed ID:9817755
'Shiga toxin and other toxins of this family can escape the endocytic pathway and reach the Golgi apparatus. To synchronize endosome to Golgi transport, Shiga toxin B-fragment was internalized into HeLa cells at low temperatures. Under these conditions, the protein partitioned away from markers destined for the late endocytic pathway