Search
Citations & References (215)
Invitrogen™
5-FAM, SE (5-Carboxyfluorescein, Succinimidyl Ester), single isomer
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.Read more
| Catalog Number | Quantity |
|---|---|
| C2210 also known as C-2210 | 10 mg |
Catalog number C2210
also known as C-2210
Price (EUR)
532,00
Each
Quantity:
10 mg
Price (EUR)
532,00
Each
Searching for superior alternatives to fluorescein? Our Alexa Fluor Dye Series offers everything you're looking for and more.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical ReactivityAmine
Label or DyeFITC (Fluorescein)
Product Type5-FAM SE
Quantity10 mg
Reactive MoietyActive Ester, Succinimidyl Ester
Shipping ConditionRoom Temperature
Label TypeClassic Dyes
Unit SizeEach
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.
Citations & References (215)
Citations & References
Abstract
Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes.
Journal:Applied and environmental microbiology
PubMed ID:9835591
In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure of the ribosome may hinder the access of oligonucleotides
Mechanism of peptide-induced mast cell degranulation. Translocation and patch-clamp studies.
Journal:J Gen Physiol
PubMed ID:9806967
Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse
Quantitative polymerase chain reaction-based homogeneous assay with fluorogenic probes to measure c-erbB-2 oncogene amplification.
Journal:Clin Chem
PubMed ID:9166227
We describe a PCR-based assay for determining c-erbB-2 oncogene amplification in breast cancer in which we use the TaqMan system. Two fluorogenic probes anneal to the target between primers for c-erbB-2 and beta-globin genes and contain both a reporter dye (6-carboxy-fluorescein) and a quencher dye (6-carboxy-tetramethyl-rhodamine). During the extension phase
DNA sequencing with dye-labeled terminators and T7 DNA polymerase: effect of dyes and dNTPs on incorporation of dye-terminators and probability analysis of termination fragments.
Journal:Nucleic Acids Res
PubMed ID:1598205
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments which were made single-stranded by digestion with T7 gene 6
Fluorescence polarization immunoassay IV. Determination of phenytoin and phenobarbital in human serum and plasma.
Journal:Clin Chem
PubMed ID:6751601
Fluorescence polarization immunoassays for phenytoin and phenobarbital in human serum or plasma are described and shown to be clinically useful. No sample pretreatment, extraction, or phase separation is required. A determination can be made with less than 20 microL of sample in 15 min, with incubation at ambient temperature. Abnormal