Search
Citations & References (39)
Invitrogen™
BODIPY™ FL C5-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosyl Phosphocholine)
BODIPY™ FL C5 sphingomyelin can be used in sphingolipid transport and metabolism studies.Read more
| Catalog Number | Quantity |
|---|---|
| D3522 | 250 μg |
Catalog number D3522
Price (EUR)
594,00
Each
Quantity:
250 μg
Price (EUR)
594,00
Each
BODIPY™ FL C5 sphingomyelin can be used in sphingolipid transport and metabolism studies.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialSphingolipids
Recommended StorageStore in freezer (-5 to -30°C) and protect from light.
Physical FormSolid
Product LineBODIPY
Quantity250 μg
Unit SizeEach
Citations & References (39)
Citations & References
Abstract
Fluorescence-based selection of gene-corrected hematopoietic stem and progenitor cells from acid sphingomyelinase-deficient mice: implications for Niemann-Pick disease gene therapy and the development of improved stem cell gene transfer procedures.
Journal:Blood
PubMed ID:9864149
'The general utility of a novel, fluorescence-based procedure for assessing gene transfer and expression has been demonstrated using hematopoietic stem and progenitor cells. Lineage-depleted hematopoietic cells were isolated from the bone marrow or fetal livers of acid sphingomyelinase-deficient mice, and retrovirally transduced with amphotropic or ecotropic vectors encoding a normal
Changes in the spectral properties of a plasma membrane lipid analog during the first seconds of endocytosis in living cells.
Journal:Biophys J
PubMed ID:8994591
'N-[5-(5, 7-dimethyl Bodipy)-1-pentanoyl]-D-erythro-sphingosylphosphorylcholine (C5-DMB-SM), a fluorescent analog of sphingomyelin, has been used in a study of the formation of very early endosomes in human skin fibroblasts. This lipid exhibits a shift in its fluorescence emission maximum from green (approximately 515 nm) to red (approximately 620 nm) wavelengths with increasing concentrations
Structural and functional regulation of tight junctions by RhoA and Rac1 small GTPases.
Journal:J Cell Biol
PubMed ID:9660866
'Tight junctions (TJ) govern ion and solute diffusion through the paracellular space (gate function), and restrict mixing of membrane proteins and lipids between membrane domains (fence function) of polarized epithelial cells. We examined roles of the RhoA and Rac1 GTPases in regulating TJ structure and function in MDCK cells using
Measurement of spontaneous transfer and transbilayer movement of BODIPY-labeled lipids in lipid vesicles.
Journal:Biochemistry
PubMed ID:9220970
'An assay was developed to study the spontaneous transfer and transbilayer movement (flip-flop) of lipid analogs labeled with the fluorescent fatty acid, 5-(5,7-dimethyl BODIPY)-1-pentanoic acid (C5-DMB-) in large unilamellar lipid vesicles comprised of 1-palmitoyl-2-oleoyl phosphatidylcholine (POPC). The assay is based on the concentration-dependent changes in fluorescence intensity that occur when
The recycling endosome of Madin-Darby canine kidney cells is a mildly acidic compartment rich in raft components.
Journal:Mol Biol Cell
PubMed ID:10930469
'We present a biochemical and morphological characterization of recycling endosomes containing the transferrin receptor in the epithelial Madin-Darby canine kidney cell line. We find that recycling endosomes are enriched in molecules known to regulate transferrin recycling but lack proteins involved in early endosome membrane dynamics, indicating that recycling endosomes are