Novex™ Tricine Mini Protein Gels, 10 to 20%, 1.0 mm
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Novex™ Tricine Mini Protein Gels, 10 to 20%, 1.0 mm
Invitrogen™

Novex™ Tricine Mini Protein Gels, 10 to 20%, 1.0 mm

Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. In this system tricine replaces glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.
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Catalog NumberWells
EC6625BOX10-well
EC66252BOX12-well
EC66255BOX15-well
Catalog number EC6625BOX
Price (EUR)
261,65
Online Exclusive
284,00
Save 22,35 (8%)
Each
Add to cart
Wells:
10-well
Price (EUR)
261,65
Online Exclusive
284,00
Save 22,35 (8%)
Each
Add to cart
Invitrogen Novex Tricine Gels provide separation of low molecular weight proteins and peptides. The Tricine system is a modification of the tris-glycine discontinuous buffer system developed by Schaegger and von Jagow (Schaegger and von Jagow, 1987) specifically for resolving peptides and low molecular weight proteins. In this system the tricine replaces the glycine in the running buffer, resulting in more efficient stacking and destacking for low molecular weight proteins and higher resolution of smaller peptides.

Features of Novex Tricine protein gels:

  • Increased resolution of proteins with molecular weights as low as 2 kDa
  • Improved compatibility with direct sequencing of proteins after transferring to PVDF
  • Minimized protein modification due to the lower pH of the tricine buffering system

Formulation

Invitrogen Tricine gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Our Tricine gels have a 4% stacking gel and do not contain SDS. The Tricine system requires SDS in sample and running buffers for best results.

Choose the right Tricine gel for your protein separation

Invitrogen Tricine gels come in three polyacrylamide concentrations of 10%, 16%, and a gradient of 10–20%. Select from our many well formats, including 10-, 12-, and 15-well. Tricine gels are formulated for denaturing gel electrophoresis applications. For optimal sample preparation, we recommend Tricine SDS Sample Buffer (LC1676) and optimal separation use Tricine SDS Running Buffer (LC1675).

For transfer of proteins to a membrane, we recommend using the Novex Tris-Glycine Transfer Buffer (LC3675) if performing a traditional wet transfer using the XCell II Blot Module (EI9051) or the Mini Blot Module (B1000). Rapid semi-dry transfer can be performed using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)XCell SureLock Mini-Cell
Gel Thickness1.0 mm
Length (Metric)8 cm
Mode of SeparationMolecular Weight
Quantity10 Gels/Box
Recommended ApplicationsDenaturing
Sample Loading VolumeUp to 25 μL
Shelf Life16 Weeks
Shipping ConditionWet Ice
Storage RequirementsStore at 2°C to 8°C. Do not freeze.
Width (Metric)8 cm
Gel Percentage10 to 20%
Gel SizeMini
Gel TypeTricine
Product LineNovex
Separation Range2.5 to 250 kDa
Separation TypeDenaturing
Wells10-well
Unit SizeEach