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Citations & References (144)
Invitrogen™
Fluorescein Diacetate (FDA)
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity,Read more
| Catalog Number | Quantity |
|---|---|
| F1303 | 1 g |
Catalog number F1303
Price (EUR)
202,00
Each
Quantity:
1 g
Price (EUR)
202,00
Each
Fluorescein diacetate (FDA) is a cell-permeant esterase substrate that can serve as a viability probe that measures both enzymatic activity, which is require to activate its fluorescence, and cell-membrane integrity, which is required for intracellular retention of their fluorescent product. Upon hydrolysis by intracellular esterases, this AM ester yields fluorescein.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Dye TypeClassic Dyes
Quantity1 g
Reagent TypeCell Tracker Compounds, Cell Labeling Reagents
Shipping ConditionRoom Temperature
Product TypeFluorescein Diacetate
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C.
Citations & References (144)
Citations & References
Abstract
Mechanistic studies of lantibiotic-induced permeabilization of phospholipid vesicles.
Journal:Biochemistry
PubMed ID:7849020
'Nisin is a cationic polycyclic bacteriocin secreted by some lactic acid bacteria. Nisin has previously been shown to permeabilize liposomes. The interaction of nisin was analyzed with liposomes prepared of the zwitterionic phosphatidylcholine (PC) and the anionic phosphatidylglycerol (PG). Nisin induces the release of 6-carboxyfluorescein and other small anionic fluorescent
Ligand/receptor internalization: a spectroscopic analysis and a comparison of ligand binding, cellular response, and internalization by human neutrophils.
Journal:J Cell Biochem
PubMed ID:6302116
'We have compared the kinetics of the responses of neutrophils to the kinetics of ligand-receptor interaction and internalization, using as a model ligand the fluoresceinated hexapeptide N-CHO-Nle-Leu-Phe-Nle-Tyr-Lys-Fluorescein (Nle, norleucine). Cellular responses, ie, membrane depolarization, enzyme (elastase) secretion, and superoxide anion (O-2) generation, are all initiated within 10 sec of the
Interaction of Mycobacterium avium-containing phagosomes with the antigen presentation pathway.
Journal:J Immunol
PubMed ID:11086039
'Pathogenic mycobacteria infect macrophages where they replicate in phagosomes that minimize contact with late endosomal/lysosomal compartments. Loading of Ags to MHC class II molecules occurs in specialized compartments with late endosomal characteristics. This points to a sequestration of mycobacteria-containing phagosomes from the sites where Ags meet MHC class II molecules.
In vitro testing of chemotherapeutic drug combinations in acute myelocytic leukaemia using the fluorometric microculture cytotoxicity assay (FMCA).
Journal:Br J Cancer
PubMed ID:8494730
'The fluorometric microculture cytotoxicity assay (FMCA) was employed for analysing the effect of different chemotherapeutic drug combinations and their single constituents in 44 cases of acute myelocytic leukaemia (AML). A large heterogeneity with respect to cell kill was observed for all combinations tested, the interactions ranging from antagonistic to synergistic
Fluorimetric quantification of cell death in monolayer cultures and cell suspensions.
Journal:J Biochem Biophys Methods
PubMed ID:1779095
'A fluorimetric assay using ethidium bromide (EB) was employed to quantify cell death in monolayer cell cultures (MA-104 cells) in situ and isolated cell suspensions (isolated colonic cells and Leishmania). Fluorescence of EB stained cells was measured with a photometer coupled to an inverted microscope for cell monolayers or in