Griess Reagent Kit, for nitrite quantitation

Citations & References (71)

Invitrogen™

Griess Reagent Kit, for nitrite quantitation

The Greiss reagent kit provides a simple and well characterized colorimetric assay for nitrites, and nitrates that have been reducedRead more

Catalog Number	Quantity
G7921	1 Kit

Catalog number G7921

Price (EUR)

348,00

Add to cart

Quantity:

1 Kit

Price (EUR)

348,00

Add to cart

The Greiss reagent kit provides a simple and well characterized colorimetric assay for nitrites, and nitrates that have been reduced to nitrites, with a detection limit of about 100 nM.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity1 Kit

Product TypeNitrite Assay Kit

Unit SizeEach

Frequently asked questions (FAQs)

How many assays can I perform with one Griess Reagent Kit, for nitrite quantitation (Cat. No. G7921)?

The number of assays that can be performed with the Griess Reagent Kit is dependent upon your assay volume.
For example:
You would use 100 µL of Component A (the Greiss reagent) for a 2 mL cuvette volume. Since we provide 25 mL in the kit, this would relate to 250 assays You would use 20 µL of Component A for a 96-well microplate, this relates to 1,250 microplate wells or about 13 microplates.

Citations & References (71)

Citations & References

Abstract

Arginase II downregulates nitric oxide (NO) production and prevents NO-mediated apoptosis in murine macrophage-derived RAW 264.7 cells.

Authors:Gotoh T, Mori M

Journal:J Cell Biol

PubMed ID:9971738

'Excess nitric oxide (NO) induces apoptosis of some cell types, including macrophages. As NO is synthesized by NO synthase (NOS) from arginine, a common substrate of arginase, these two enzymes compete for arginine. There are two known isoforms of arginase, types I and II. Using murine macrophage-like RAW 264.7 cells, ... More

Augmentation or inhibition of IFN-gamma-induced MHC class II expression by lipopolysaccharides. The roles of TNF-alpha and nitric oxide, and the importance of the sequence of signaling.

Authors:Sicher SC, Chung GW, Vazquez MA, Lu CY

Journal:J Immunol

PubMed ID:7499872

'MHC class II expression on macrophages is one determinant of Ag presentation and the vigor of CD4+ T cell immunity. We show that LPS may either inhibit or augment IFN-gamma-induced MHC class II on macrophages depending on the sequence of the IFN-gamma and LPS signals. LPS inhibited MHC class II ... More

Enzymatic microtiter plate-based nitrate detection in environmental and medical analysis.

Authors:Borcherding H, Leikefeld S, Frey C, Diekmann S, Steinrücke P

Journal:Anal Biochem

PubMed ID:10860492

'Our microtiter plate assay is based on the enzymatic reduction of nitrate by dissimilatory nitrate reductase from Pseudomonas stutzeri [EC 1.7.99.4]. Exogenous redox mediators like methyl viologen, methylene blue, and cibachron blue were applied to reduce nitrate reductase. Concentrations of 0.02-0.9 mM nitrate can be detected with +/-6% standard deviation, ... More

Measurement of nitrite and nitrate in biological fluids by gas chromatography-mass spectrometry and by the Griess assay: problems with the Griess assay--solutions by gas chromatography-mass spectrometry.

Authors:Tsikas D, Gutzki FM, Rossa S, Bauer H, Neumann C, Dockendorff K, Sandmann J, Frölich JC

Journal:Anal Biochem

PubMed ID:9025936

'Assay methods based on the Griess reaction are frequently used to measure nitrite and nitrate in urine, plasma, and other biological fluids. With minor exceptions, careful attention has not been paid in extending the Griess assay from aqueous solutions to biological fluids, In the present study, parallel measurements of nitrite ... More

Pyrimidinoceptor-mediated potentiation of inducible nitric-oxide synthase induction in J774 macrophages. Role of intracellular calcium.

Authors:Chen BC, Chou CF, Lin WW

Journal:J Biol Chem

PubMed ID:9792689

'We have shown that, in murine J774 macrophages, binding of UTP to pyrimidinoceptors stimulates phosphoinositide (PI) breakdown and an increase in [Ca2+]i. In this study, UTP modulation of the expression of inducible nitric-oxide synthase (iNOS) was investigated. Although UTP alone had no effect, stimulation of J774 cells with a combination ... More

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