Thermo Scientific Chemicals

Ponceau S, Electrophoresis Grade, Thermo Scientific Chemicals

Catalog number: J60744.18
50 g, Each
Thermo Scientific Chemicals

Ponceau S, Electrophoresis Grade, Thermo Scientific Chemicals

Catalog number: J60744.18
50 g, Each
Quantity
Catalog number: J60744.18
also known as J60744-18
Price (EUR)
Quantity
-

Chemical Identifiers

CAS
6226-79-5
IUPAC Name
tetrasodium (4Z)-3-oxo-4-(2-{2-sulfonato-5-[2-(4-sulfonatophenyl)diazen-1-yl]phenyl}hydrazin-1-ylidene)-3,4-dihydronaphthalene-2,7-disulfonate
Molecular Formula
C22H12N4Na4O13S4
InChI Key
LZYGEJPERQQCIY-STNZDNLRSA-J
SMILES
[Na+].[Na+].[Na+].[Na+].[O-]S(=O)(=O)C1=CC=C(C=C1)N=NC1=CC=C(C(N\N=C2/C(=O)C(=CC3=CC(=CC=C23)S([O-])(=O)=O)S([O-])(=O)=O)=C1)S([O-])(=O)=O
Appearance (Color)
Red-brown to dark brown
Absorbance
1%/1cm at lambda max. 519.4 nm: ≥460
Form
Powder
Loss on Drying
≤5.0%
Assay (Dye)
Dye Content: ≥80.0%

Description

Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

Applications
Ponceau S dye is used to make a stain for rapid reversible staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It is also suitable for electrophoresis, azo dye for reversible protein staining. This stain can also be used as a fiducial marker in mass spectrometric imaging.

Solubility
Soluble in water (10 g/L) at 20°C. Insoluble in ether.

Notes
Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Keep away fom strong oxidizing agents.
RUO – Research Use Only

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    Safety and Handling


    Classification of the substance or mixture
    CLP classification - Regulation(EC) No 1272/2008
    Skin Corrosion/Irritation
    Category 2
    Serious Eye Damage/Eye Irritation
    Category 2
    Specific target organ toxicity - (single exposure)
    Category 3
    Label Elements
    Signal Word

    Warning

    Hazard Statements

    H315 - Causes skin irritation

    H319 - Causes serious eye irritation

    H335 - May cause respiratory irritation

    Precautionary Statements

    P264 - Wash face, hands and any exposed skin thoroughly after handling

    P280 - Wear protective gloves/protective clothing/eye protection/face protection

    P304 + P340 - IF INHALED: Remove person to fresh air and keep comfortable for breathing

    P312 - Call a POISON CENTER or doctor if you feel unwell

    P337 + P313 - If eye irritation persists: Get medical advice/attention