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3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%, Thermo Scientific Chemicals
Catalog number: J62216.06
5 g, Each
Thermo Scientific Chemicals
3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%, Thermo Scientific Chemicals
Catalog number: J62216.06
5 g, Each
Quantity
Catalog number: J62216.06
also known as J62216-06
Price (EUR)
75,87 Online price
84,30
Each
Quantity
-
Chemical Identifiers
CAS
868272-85-9
IUPAC Name
[1,1'-biphenyl]-3,3',4,4'-tetramine tetrahydrochloride
Molecular Formula
C12H18Cl4N4
InChI Key
KJDSORYAHBAGPP-UHFFFAOYSA-N
SMILES
Cl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1
Specifications
Specification SheetAssay (unspecified)
≥97%
Form
Powder or crystalline powder
Appearance (Color)
Pale grey to grey to dark brown or brown-purple
Loss on Drying
≤10.0 % (120°C/constant weight)
Description
3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.
This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.
General Description
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is an organic compound used as a peroxidase substrate
Application
• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is commonly used for immunoblotting and immunohistochemical staining techniques
• It has also been used for the histological stain to measure the peroxidase activity. The interaction between 3,3'-Diaminobenzidine tetrahydrochloride hydrate and peroxidase leads to the formation of a visible insoluble product. Therefore, it has been applied to measure glutamate decarboxylase activity in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces
• It is used as a chromogen for detecting the antigen-antibody complex
• This compound is also used in several laboratory applications, including electron and light microscope, and FISH (fluorescence in situ hybridization)
RUO – Research Use Only
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Safety and Handling
Classification of the substance or mixture
CLP classification - Regulation(EC) No 1272/2008
Germ Cell Mutagenicity
Category 2
Carcinogenicity
Category 1B
Chronic aquatic toxicity
Category 3
Label Elements
Signal Word
Danger
Hazard Statements
H341 - Suspected of causing genetic defects
H350 - May cause cancer
H412 - Harmful to aquatic life with long lasting effects
Precautionary Statements
P201 - Obtain special instructions before use
P280 - Wear protective gloves/protective clothing/eye protection/face protection
P308 + P313 - IF exposed or concerned: Get medical advice/attention
Additional EU labelling
Restricted to professional users