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3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%
3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%
3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%
Thermo Scientific Chemicals

3,3'-Diaminobenzidine tetrahydrochloride hydrate, 97%

3,3'-Diaminobenzidine tetrahydrochloride hydrate, CAS # 868272-85-9, is a peroxidase substrate that is commonly used for immunoblotting and immunohistochemical staining techniques. | CAS: 868272-85-9 | C12H18Cl4N4 | 360.10 g/mol
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Catalog number J62216.09
also known as J62216-09
Price (EUR)
234,00
Each
Quantity:
10 g
Request bulk or custom format
Price (EUR)
234,00
Each
Chemical Identifiers
CAS868272-85-9
IUPAC Name[1,1'-biphenyl]-3,3',4,4'-tetramine tetrahydrochloride
Molecular FormulaC12H18Cl4N4
InChI KeyKJDSORYAHBAGPP-UHFFFAOYSA-N
SMILESCl.Cl.Cl.Cl.NC1=C(N)C=C(C=C1)C1=CC(N)=C(N)C=C1
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SpecificationsSpecification SheetSpecification Sheet
Assay (unspecified)≥97%
FormPowder or crystalline powder
Appearance (Color)Pale grey to grey to dark brown or brown-purple
Loss on Drying≤10.0 % (120°C/constant weight)
3,3?-diaminobenzidine-tetrahydrochloride-dihydrate (DAB) was used as a chromogen, for detecting the antigen-antibody complex. It is one of the most potent marker being used in immunochemistry, mostly at the electron and light microscope levels. It is used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific Chemicals.

General Description

• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is an organic compound used as a peroxidase substrate

Application

• 3,3'-Diaminobenzidine tetrahydrochloride hydrate is commonly used for immunoblotting and immunohistochemical staining techniques

• It has also been used for the histological stain to measure the peroxidase activity. The interaction between 3,3'-Diaminobenzidine tetrahydrochloride hydrate and peroxidase leads to the formation of a visible insoluble product. Therefore, it has been applied to measure glutamate decarboxylase activity in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces

• It is used as a chromogen for detecting the antigen-antibody complex

• This compound is also used in several laboratory applications, including electron and light microscope, and FISH (fluorescence in situ hybridization)

RUO – Research Use Only

General References:

  1. Roschzttardtz, H.; Grillet, L.; Isaure, M.-P.; Conejero, G.; Ortega, R.; Curie, C.; Mari, S. The Plant Cell Nucleolus as a Hot Spot for Iron. The Journal of Biological Chemistry. 2011, 286, (32), 27863-27866.
  2. JK Stille.; JR Williamson. Polyquinoxalines. Journal of Polymer Science Part A. 1964, 2, (9), 3867-3875.