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Thermo Scientific™
GeneJET Genomic DNA Purification Kit
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA fromRead more
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| Catalog Number | Quantity |
|---|---|
| K0722 | 250 Preps |
| K0721 | 50 Preps |
Catalog number K0722
Price (EUR)
892,00
Each
Quantity:
250 Preps
Price (EUR)
892,00
Each
Thermo Scientific GeneJET Genomic DNA Purification Kit is designed for rapid and efficient purification of high quality genomic DNA from various mammalian cell culture and tissue samples, whole blood, bacteria and yeast.
The kit utilizes silica-based membrane technology in the form of a convenient spin column, eliminating the need for expensive resins, toxic phenol-chloroform extractions, or time-consuming alcohol precipitation.
The standard procedure takes less than 20 minutes following cell lysis and yields purified DNA greater than 30 kb in size. Isolated DNA can be used directly in PCR, Southern blotting and enzymatic reactions.
Highlights
• Universal—can be used for both cell and tissue samples from a wide range of sources
• Efficient—high yields of high molecular weight genomic DNA
• Fast—20 minute procedure following cell lysis
• Convenient—spin columns are capped and assembled with collection tubes
Applications
Isolated genomic DNA is ideal for use in common molecular biology procedures, including:
• PCR and qPCR
• FastDigest and conventional restriction digestion
• Southern blotting
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Column TypeSpin Column
Elution Volume200 μL
Final Product TypegDNA
For Use With (Application)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Quantity250 Preps
Sample TypeBacteria, Cells, Rodent Tail, Tissue, Whole Blood, Yeast, Buccal Samples
ScaleMini
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤109 cells
Cells: ≤106
Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)
Tissue: ≤20 mg
Whole Blood: ≤200 μL
Yeast: ≤108 cells
Cells: ≤106
Rodent Tail: 0.6 cm (rat), 0.5 cm (mouse)
Tissue: ≤20 mg
Whole Blood: ≤200 μL
Yeast: ≤108 cells
Test Time20 min. (After sample homogenization)
Yield≤30 μg
Isolation TechnologySilica Spin Column
Unit SizeEach
Contents & Storage
• 5 x 1.2 mL Proteinase K Solution; store at room temperature until opened, –20°C after opening
• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening
• 55 mL Digestion Solution; room temperature
• 2 x 60 mL Lysis Solution; room temperature
• 40 mL Wash Buffer I (concentrated); room temperature
• 40 mL Wash Buffer II (concentrated); room temperature
• 150 mL Elution Buffer; room temperature
• 250 GeneJET Purification Columns with Collection Tubes; room temperature
• 250 Collection Tubes; room temperature
• 5 x 1 mL RNase A Solution; store at room temperature until opened, –20°C after opening
• 55 mL Digestion Solution; room temperature
• 2 x 60 mL Lysis Solution; room temperature
• 40 mL Wash Buffer I (concentrated); room temperature
• 40 mL Wash Buffer II (concentrated); room temperature
• 150 mL Elution Buffer; room temperature
• 250 GeneJET Purification Columns with Collection Tubes; room temperature
• 250 Collection Tubes; room temperature