NeuroTrace™ 435/455 Blue Fluorescent Nissl Stain

Citations & References (28)

Invitrogen™

NeuroTrace™ 435/455 Blue Fluorescent Nissl Stain

Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Composed of ribosomal RNARead more

Catalog Number	Quantity
N21479	1 mL

Catalog number N21479

Price (EUR)

423,00

Add to cart

Quantity:

Price (EUR)

423,00

Add to cart

Nissl staining is a standard histological method for visualizing neurons in the brain and spinal cord. Composed of ribosomal RNA associated with the rough endoplasmic reticulum in neuronal perikarya and dendrites, the Nissl substance redistributes within the cell body in injured or regenerating neurons, providing a marker for the physiological state of the neuron. Our NeuroTrace 435/455 blue-fluorescent Nissl stain is selective for the Nissl substance characteristic of neurons and provides more sensitivity than traditional histological dyes like toluidine blue or cresyl violet.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorBlue

Detection MethodFluorescence

For Use With (Equipment)Fluorescence Microscope

Product TypeNissl Stain

Quantity1 mL

Shipping ConditionRoom Temperature

Sub Cellular LocalizationNissl Bodies

Excitation/Emission435/455 nm

Product LineNEUROTRACE

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am labeling brain cryosections with a NeuroTrace Nissl Stain. Is this compatible with antibody labeling?

Yes. We have done this successfully with an anti-GFAP primary and an Alexa Fluor secondary antibody. We would recommend labeling with the primary and secondary antibodies first, then following up with the standard NeuroTrace Nissl Stain protocol.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I can use the NeuroTrace Nissl stains for staining glia or other cell types. What can I do to improve the staining so that it is more selective for neurons?

Our NeuroTrace Nissl stains label the Nissl substance which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells. These dyes are not completely specific for neurons, but will selectively stain neurons based on their high level of protein synthesis. In some cases they may show staining of other cell types such as glia, so you may need to decrease the staining concentration to obtain more selective neuronal labeling. We suggest dilutions in the range of 20- to 300-fold for neuronal staining.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can the NeuroTrace Nissl stains be used on paraffin sections?

We have only tested them on mouse brain cryosections, however, there is at least one citation describing their use on paraffin tissue sections (Michelle L. Schlief, Ann Marie Craig, and Jonathan D. Gitlin. NMDA Receptor Activation Mediates Copper Homeostasis in Hippocampal Neurons. The Journal of Neuroscience, January 5, 2005, 25(1):239 - 246).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What are the NeuroTrace Nissl stains?

The dyes are proprietary, however they are stains that label the Nissl substance, which is composed of ribosomal RNA associated with the rough endoplasmic reticulum and is present in high amounts in neuronal cells.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What products do you have for neuronal tracing?

Please check out this web page (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-tracing-tracking-and-morphology/neuronal-tracing.html) for details.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (28)

Citations & References

Abstract

Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury.

Authors:Bareyre FM, Kerschensteiner M, Misgeld T, Sanes JR

Journal:Nat Med

PubMed ID:16286922

'The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here, ... More

Direction-selective retinal ganglion cells arise from molecularly specified multipotential progenitors.

Authors:De la Huerta I, Kim IJ, Voinescu PE, Sanes JR,

Journal:Proc Natl Acad Sci U S A

PubMed ID:23045641

'Single progenitors can give rise to any and all of the main retinal cell types: photoreceptors, interneurons (horizontal, bipolar, and amacrine cells), retinal ganglion cells (RGCs), and glia. Many of these types are divisible into multiple functionally, structurally, and molecularly distinct subtypes (e.g., ~25 for RGCs). It remains unknown when ... More

In vivo imaging reveals a phase-specific role of STAT3 during central and peripheral nervous system axon regeneration.

Authors:Bareyre FM, Garzorz N, Lang C, Misgeld T, Büning H, Kerschensteiner M,

Journal:Proc Natl Acad Sci U S A

PubMed ID:21447717

'In the peripheral nervous system (PNS), damaged axons regenerate successfully, whereas axons in the CNS fail to regrow. In neurons of the dorsal root ganglia (DRG), which extend branches to both the PNS and CNS, only a PNS lesion but not a CNS lesion induces axonal growth. How this differential ... More

A synaptic basis for auditory-vocal integration in the songbird.

Authors:Bauer EE, Coleman MJ, Roberts TF, Roy A, Prather JF, Mooney R,

Journal:J Neurosci

PubMed ID:18256272

'Songbirds learn to sing by memorizing a tutor song that they then vocally mimic using auditory feedback. This developmental sequence suggests that brain areas that encode auditory memories communicate with brain areas for learned vocal control. In the songbird, the secondary auditory telencephalic region caudal mesopallium (CM) contains neurons that ... More

CO2-sensitive preinspiratory neurons of the parafacial respiratory group express Phox2b in the neonatal rat.

Authors:Onimaru H, Ikeda K, Kawakami K,

Journal:J Neurosci

PubMed ID:19036978

'Phox2b protein is a specific marker for neurons in the parafacial region of the ventral medulla, which are proposed to play a role in central chemoreception and postnatal survival. Mutations of PHOX2B cause congenital central hypoventilation syndrome. However, there have been no reports concerning electrophysiological characteristics of these Phox2b-expressing neurons ... More

View all NeuroTrace™ 435/455 Blue Fluorescent Nissl Stain citations