Quant-iT™ OliGreen™ ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent
Use 96- and 384-well Microplates for Fluorescence-based Assays with Quant-iT assays for optimal results
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Citations & References (52)
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Quant-iT™ OliGreen™ ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent

Quant-iT™ OliGreen ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution.
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Catalog NumberQuantityProduct Type
O75821 mLQuant-iT OliGreen ssDNA Rgnt
O114921 kitQuant-iT OliGreen ssDNA Kit
Catalog number O7582
Price (EUR)
980,00
Each
Add to cart
Quantity:
1 mL
Product Type:
Quant-iT OliGreen ssDNA Rgnt
Price (EUR)
980,00
Each
Add to cart
Quantify ssDNA with ease using the Quant-iT OliGreen ssDNA Assay kit and reagent. This assay kit and reagent enable sensitive quantitation of single-stranded DNA without the need for a highly concentrated sample. As little as 100 pg/mL can be quantitated using the assay kit or Quant-iT OliGreen ssDNA Reagent.
Quantitate ssDNA and other oligonucleotides accurately and without the need for a highly concentrated sample with Quant-iT OliGreen ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent. Quant-iT OliGreen ssDNA Assay Kits contain an ultrasensitive, green-fluorescent nucleic acid stain for quantitating oligonucleotides and single-stranded DNA (ssDNA) in solution. The Quant-iT™ OliGreen™ ssDNA Assay Reagent enables researchers to quantify as little as 1 ng/mL oligonucleotide or ssDNA (200 pg in a 200 μL assay volume) with a fluorescence microplate reader using fluorescein excitation and emission wavelengths. This sensitivity exceeds that achieved with absorbance methods by 10,000-fold.

In addition to the dye, the OliGreen ssDNA Assay Kit includes buffer and an oligonucleotide standard. The OliGreen reagent is also available as the stand-alone Quant-iT OliGreen ssDNA Reagent.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200 assays (2 mL assay volume)
Product LineOLIGREEN, Quant-iT
Product TypeQuant-iT OliGreen ssDNA Rgnt
Quantitation Range200 pg to 2 μg
Quantity1 mL
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach

Frequently asked questions (FAQs)

Why am I getting negative fluorescence values with my Qubit Assays?

Negative fluorescence is a physical impossibility. It is an artifact from software autocorrecting for background signal. This means your reader is picking up and subtracting out background light at the cost of your data. Make sure to do a buffer-only control and assess the type of signal. You may need to switch to a different plate.

I have a Quant-iT DNA Kit and want to use it for the Qubit Fluorometer. Can I?

Yes, the manual has directions for this application. You will use the 0 ng/µL lambda dsDNA HS standard to generate Standard #1. You will prepare a dilution of the 10 ng/µL lambda dsDNA HS standard to generate Standard #2. You then prepare the samples and compare them to this 2-point standard curve. The Quant-iT dsDNA BR Kit can be used in a similar manner.

What is the useful pH range for Quant-iT DNA kits?

The buffer included in the kit should assure the proper pH range, even if your DNA is at a pH outside of this range, since at least a 10-fold excess of kit buffer over sample is used in the assay.

I'm trying to quantify some DNA labeled with a fluorophore. Will this work?

PicoGreen dye and other fluorescence-based quantification reagents are not recommended for quantifying dye-conjugated nucleic acids. The attached dye molecules can interfere with either binding and/or fluorescence output of the quantification reagents.

Does DNA length have an effect on the dsDNA assays?

Strands that are roughly in the 20-mer range or shorter show a lower level of signal. For dsDNA samples that are composed of mostly short strands, the reagent may still be used, but one should use a dsDNA standard that is of comparable length as the sample.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Quantification Support Center.

View all Quant-iT™ OliGreen™ ssDNA Assay Kit and Quant-iT OliGreen ssDNA Reagent FAQs

Citations & References (52)

Citations & References
Abstract
Discovery and characterization of a thermostable bacteriophage RNA ligase homologous to T4 RNA ligase 1.
Authors:Blondal T, Hjorleifsdottir SH, Fridjonsson OF, Aevarsson A, Skirnisdottir S, Hermannsdottir AG, Hreggvidsson GO, Smith AV, Kristjansson JK
Journal:Nucleic Acids Res
PubMed ID:14654700
Thermophilic viruses represent a novel source of genetic material and enzymes with great potential for use in biotechnology. We have isolated a number of thermophilic viruses from geothermal areas in Iceland, and by combining high throughput genome sequencing and state of the art bioinformatics we have identified a number of ... More
Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease.
Authors:Ott SJ, Musfeldt M, Wenderoth DF, Hampe J, Brant O, Fölsch UR, Timmis KN, Schreiber S
Journal:Gut
PubMed ID:15082587
BACKGROUND AND AIMS: The intestinal bacterial microflora plays an important role in the aetiology of inflammatory bowel disease (IBD). As most of the colonic bacteria cannot be identified by culture techniques, genomic technology can be used for analysis of the composition of the microflora. PATIENTS AND METHODS: The mucosa associated ... More
Smooth muscle overexpression of IGF-I induces a novel adaptive response to small bowel resection.
Authors:Knott AW, Juno RJ, Jarboe MD, Profitt SA, Erwin CR, Smith EP, Fagin JA, Warner BW
Journal:Am J Physiol Gastrointest Liver Physiol
PubMed ID:15142831
'Prior studies of intestinal adaptation after massive small bowel resection (SBR) have focused on growth factors and their effects on amplification of the gut mucosa. Because adaptive changes have also been described in intestinal smooth muscle, we sought to determine the effect of targeted smooth muscle growth factor overexpression on ... More
The histone deacetylase inhibitor trichostatin A alters the pattern of DNA replication origin activity in human cells.
Authors:Kemp MG, Ghosh M, Liu G, Leffak M,
Journal:Nucleic Acids Res
PubMed ID:15653633
'Eukaryotic chromatin structure limits the initiation of DNA replication spatially to chromosomal origin zones and temporally to the ordered firing of origins during S phase. Here, we show that the level of histone H4 acetylation correlates with the frequency of replication initiation as measured by the abundance of short nascent ... More
Fast separation of single-stranded oligonucleotides by capillary electrophoresis using OliGreen as fluorescence inducing agent.
Authors:Zhang J, Liang D, He W, Wan F, Ying Q, Chu B
Journal:Electrophoresis
PubMed ID:16315172
'The fast separation of oligonucleotide (oligos) sizing marker by CE using OliGreen and including effects due to the concentration of separation medium and urea denaturant is presented. OliGreen dye is found to be more sensitive than ethidium bromide (by a factor of about 6 based on S/N considerations) for the ... More
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