Desoxycholate Citrate Agar (Hynes)

Isolate and differentiate Salmonella and Shigella species from clinical samples easily and with improved selectivity with Thermo Scientific™ Desoxycholate Citrate Agar (Hynes Modification) media plates.

An improved medium, based on the Hynes¹ modification of Leifson’s medium², Desoxycholate Citrate Agar (Hynes Modification) makes the isolation of salmonellae and shigellae easier.

Easier colony selection: improved medium gives larger and more numerous colonies of Shigella species which can easily be picked off and emulsified in saline for slide agglutination tests
Improved selectivity: more inhibitory to coliforms and Proteus species than the original Desoxycholate Citrate Agar (Part No. CM0035B)
Ready-to-use: convenience of prepared media.

The medium is pale pink, due to the inclusion of Neutral Red indicator, and clear. Lactose fermenting organisms produce pink colonies and may be surrounded by a zone of precipitated desoxycholic acid. The colonies of non-lactose fermenters are colorless, and, due to their alkaline reaction, they are surrounded by a clear orange-yellow zone of medium. Escherichia coli survives on the medium even though it does not usually grow; therefore, colonial purity should be established by subculture on to a differential but less inhibitory medium, e.g., MacConkey Agar (Part No. CM0007B).

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