SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.
SYPRO Ruby Protein Gel Stain features include: • Convenient—ready-to-use formulation • High sensitivity—detection to 0.25 to 1 ng • Effective staining for 1D and 2D gels • Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain • Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry
SYPRO Orange and SYPRO Red protein gel stain features include: • Sensitive—detection to 4 to 8 ng • Fast—rapid staining time of 10–60 min • Best for 1D gels • SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm • SYPRO Red: Ex: 300, 550 nm, Em: 630 nm
SYPRO Tangerine Protein Gel Stain features include: • Sensitive—detection to 4 to 8 ng • Fast—rapid staining time of 10 to 60 minutes • Best for 1D gels • Does not require fixation with acetic acid • Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry
For Research Use Only. Not for use in diagnostic procedures.