CyQUANT™ Cytotoxicity Assay Kit (G6PD Release Assay)

CyQUANT cytotoxicity assays provide a simple and reliable method to assess chemical or cell-mediated cellular toxicity. The CyQUANT LDH Cytotoxicity Assays and the CyQUANT Cytotoxicity Assay (G6PD Release Assay) use colorimetric- or fluorescence-based detection to measure the release of cytosolic enzymes (LDH or G6PD) from damaged cells. These cytotoxicity assays are optimized for use on microplate readers and can be used for continuous monitoring of cell health over time since cell lysis is not required, making them versatile tools for high-throughput screening and drug discovery applications.

With the CyQuant G6PD Release Assay (V23111), damaged and dying cells release glucose 6-phosphate dehydrogenase (G6PD) into the surrounding medium. G6PD is detected through a two-step enzymatic process via oxidation of glucose 6-phosphate by G6PD to generate NADPH, which leads to the reduction of resazurin by diaphorase to red-fluorescent resorufin. This assay results in lower background signals than are typically observed in LDH assays, offering increased sensitivity. It can detect as few as 500 cells and is more sensitive than LDH release assays.

Features of the CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay)

• High sensitivity—detects as few as 500 cells, offering greater sensitivity than LDH release assays
• Specific detection—measures glucose 6-phosphate dehydrogenase (G6PD) release from damaged and dying cells
• Low background signals—produces lower background fluorescence compared to LDH release assays
• Accurate and reliable—the fluorescence signal (ex/em 563/587 nm) is proportional to the number of dead cells, ensuring accurate cytotoxicity measurement