Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 4 to 12%
Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 4 to 12%
Invitrogen™

Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 4 to 12%

The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents needed to begin using the Mini Gel Tank.
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Catalog NumberWells
XP0412A10-well
XP0412B12-well
XP0412C15-well
Catalog number XP0412A
Price (EUR)
684,00
1 kit
Add to cart
Wells:
10-well
Price (EUR)
684,00
1 kit
Add to cart
The Novex Tris-Glycine Welcome Pack provides all of the necessary Novex Tris-glycine gels, buffers, and reagents you need to begin using the Mini Gel Tank. The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

The Novex Tris-Glycine Welcome Pack contains:
• Mini Gel Tank (A25977)
• Novex Tris-Glycine Mini Gels, WedgeWell format (2 boxes, 20 gels)
• Novex Tris-Glycine SDS Running Buffer, 10X (LC2675)
• Novex Tris-Glycine SDS Sample Buffer, 2X (LC2676)
• NuPAGE Sample Reducing Agent, 10X (NP0004)
• PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa (26619)

About the Mini Gel Tank
The Mini Gel Tank is compatible with all Novex and NuPAGE mini gels. Each Mini Gel Tank can accommodate up to two gels per run. The unique tank design enables convenient, side-by-side gel loading and enhanced viewing during use. Run times may vary depending on buffer conditions and power supplies being used.

About Novex Tris-Glycine gels, WedgeWell format
Novex Tris-Glycine mini gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis, allowing the use of Laemmli sample and running buffers. They enable consistent performance and separation of a wide range of proteins into well-resolved bands. Novex Tris-Glycine gels, WedgeWell format, have innovative wedge-shaped wells that allow up to two times more sample load volume than other 1.0 mm-thick gels. The wedge-shaped wells also have larger openings, making sample loading easier.

For transferring your proteins to a membrane, we recommend using for Novex Tris-Glycine Native Running Buffer (LC2672) or Novex Tris-Glycine Transfer Buffer (LC3675) for traditional wet transfer using the Mini Blot Module (B1000). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Gel Thickness1.0 mm
Kit ContentsPageRuler™ Plus Prestained Protein Ladder
Product LineNovex, WedgeWell
Quantity1 Welcome Pk. kit
Shipping ConditionApproved for shipment at room temperature or on wet ice
For Use With (Equipment)Mini Gel Tank
Gel Percentage4 to 12%
Gel SizeMini
Gel TypeTris-Glycine
Separation Range30 to 250 kDa
Wells10-well
Unit Size1 kit

Frequently asked questions (FAQs)

Is it okay to use protein gels past their expiration date?

We do not recommend using gels past their expiration date because over time, the polyacrylamide hydrolyzes to acrylic acid and ammonia and this will affect the resolution of the proteins. Breakdown of polyacrylamide matrix is identified by:
- Ghost bands and doublets, seen first in the high molecular weight proteins
- Smiling of dye front across the gel, with bands in outer lanes becoming very slanted - proteins run slower there due to change in pH and pore size over time.


Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Can I run your protein gels overnight?

This is not really recommended, but it is always possible to increase run time by lowering the voltage of the run. In general, the relationships are linear - i.e., decreasing voltage by half will double the run time.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Do I need to increase the voltage when I run a 1.5 mm protein gel versus a 1.0 mm gel?

If you are running the gel at constant voltage, you do not need to increase the voltage regardless of the thickness of the gel.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Why do you recommend running your protein gels at constant voltage?

Using constant voltage allows the current and power to decrease during the run, providing a safety margin in case of a break in the system. Having lower power is a safety benefit and will also decrease the chances of experiencing an overheating of the gel. Further, the constant voltage setting does not need adjustment to account for differences in number or thickness of gels being run.

Find additional tips, troubleshooting help, and resources within ourProtein Gel 1D Electrophoresis Support Center.

I used one of your protein standards for a western transfer and noticed that some of the lower-molecular weight protein bands passed through the membrane. How can I resolve this issue?

- Decrease voltage, current or length of transfer time
- Make sure that the methanol concentration in the transfer buffer is proper; use a methanol concentration of 10-20% methanol removes the SDS from SDS-protein complexes and improves the binding of protein to the membrane.
- Make sure that the SDS concentration (if added) in the transfer buffer is proper, don't use more than 0.02-0.04% SDS. Using too much SDS can prevent binding of proteins to the membrane.
- Check the pore size of the membrane and the size of the target protein. Proteins smaller than 10 kDa will easily pass through a 0.45 µm pore size membrane. If proteins smaller than 10 kDa are of interest, it would be better to use a 0.2 µm pore size membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Novex™ WedgeWell™ Tris-Glycine Welcome Pack, 4 to 12% FAQs