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Conjugués pHrodo™ BioParticles™ pour la phagocytose et kit de phagocytose, pour la cytométrie en flux
Citations et références (8)
Invitrogen™

Conjugués pHrodo™ BioParticles™ pour la phagocytose et kit de phagocytose, pour la cytométrie en flux

• Détectez spécifiquement la phagocytose et l’endocytose avec un colorant fluorogénique sensible au pH - distinguez l’endocytose à partir deAfficher plus
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RéférenceType de produitEspècesColor
A10025Kit de phagocytoseE. coli
P35360ConjuguéE. coli
P35361ConjuguéE. coli
A10010ConjuguéS. aureus
P35367ConjuguéS. aureus
P35365ConjuguéS. cerevisiae
Référence A10025
Prix (EUR)
898,65
Online Exclusive
1 198,00
Économisez 299,35 (25%)
Each
Type de produit:
Kit de phagocytose
Espèces:
E. coli
Prix (EUR)
898,65
Online Exclusive
1 198,00
Économisez 299,35 (25%)
Each
• Détectez spécifiquement la phagocytose et l’endocytose avec un colorant fluorogénique sensible au pH - distinguez l’endocytose à partir de particules adhérentes et extracellulaires
• Réduction de la variabilité du signal et meilleure synchronisation dans les expériences sensibles - pas besoin de
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
Spécifications
DescriptionKit de phagocytose pHrodo™ Red E. coli BioParticles™ pour la cytométrie en flux
Méthode de détectionFluorescence
Type de colorantpHrodo™ Rouge
FormatFlacon(s)
Quantité100 dosages
Conditions d’expéditionTempérature ambiante
EspècesE. coli
CouleurRouge
Emission488 nm
À utiliser avec (application)Analyse cellulaire
À utiliser avec (équipement)Cytomètre en flux
Gamme de produitsBioParticles, pHrodo
Type de produitKit de phagocytose
Unit SizeEach
Contenu et stockage
• Tampon de lyse, 10 ml
• Tampon B, 200 ml
• Tampon de lavage, 200 ml
• pHrodo™ Red E. coli BioParticles™, 1 flacon de produit lyophilisé

Conserver au réfrigérateur (2–8°C) à l’abri de la lumière.

Foire aux questions (FAQ)

I am performing a phagocytosis assay of macrophages engulfing pHrodo-labeled bacteria. What do you recommend for fixation after the phagocytosis?

pHrodo is relatively non-fluorescent until it enters the acidic phagosome, at which point its fluorescence increases. If you fix the sample, the pHrodo will only reflect the pH of the buffer the cells are in, and not the pH of the phagosome. For this reason, we do not recommend fixing samples. If you want to see how many cells engulfed the labeled bacteria, fix the cells and then place the fixed cells in an acidic buffer for the assay.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Are the Invitrogen BioParticles products sterile?

While the bacteria have been attenuated with formaldehyde and alcohol desiccation, the BioParticles products are not considered sterile, and we do not recommend incubation of more than 4 hours. This applies to all of our dye-labeled (pHrodo, Alexa Fluor, etc.) and unlabeled BioParticles products.

What is the concentration of bacterial particles for pHrodo BioParticles Conjugates for Phagocytosis?

The bacterial particles per weight for pHrodo BioParticles Conjugates for Phagocytosis, is as follows:

pHrodo E. Coli BioParticles Conjugate for Phagocytosis (Cat. Nos. P35360, P35361, A10025) contains 3 x 108 particles/mg.

pHrodo S. aureus BioParticles Conjugate for Phagocytosis (Cat. Nos. A10010, P35367) contains 3 x 108 particles/mg.

pHrodo Zymosan BioParticles Conjugate for Phagocytosis (Cat. No. P35365) contains 2 x 107 particles/mg.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store the pHrodo BioParticles included within pHrodo BioParticles Phagocytosis kits for flow cytometry?

The pHrodo BioParticles are supplied within the kits in lyophilized form. We recommend storing the pHrodo BioParticles vial(s) at 2-8 degrees C, desiccated and protected from light. Once reconstituted, the pHrodo BioParticles can be stored at 2-8 degrees C for several weeks, as long as sodium azide is added to a final concentration of 2 mM. If no sodium azide is added, the cell suspension needs to be used right away or on the same day to avoid contamination.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

What is the type of bond that attaches the dyes to the BioParticles probes?

We use amine-reactive dyes to covalently attach fluorescent dyes to all of our BioParticles probes such as the Escherichia coli (K-12 strain) BioParticles probes, Staphylococcus aureus (Wood strain without protein A) BioParticles, and the Zymosan A (S. cerevisiae) BioParticles probes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Conjugués pHrodo™ BioParticles™ pour la phagocytose et kit de phagocytose, pour la cytométrie en flux FAQs

Citations et références (8)

Citations et références
Abstract
SLAM is a microbial sensor that regulates bacterial phagosome functions in macrophages.
Authors:Berger SB, Romero X, Ma C, Wang G, Faubion WA, Liao G, Compeer E, Keszei M, Rameh L, Wang N, Boes M, Regueiro JR, Reinecker HC, Terhorst C,
Journal:Nat Immunol
PubMed ID:20818396
'Phagocytosis is a pivotal process by which macrophages eliminate microorganisms after recognition by pathogen sensors. Here we unexpectedly found that the self ligand and cell surface receptor SLAM functioned not only as a costimulatory molecule but also as a microbial sensor that controlled the killing of gram-negative bacteria by macrophages. ... More
Glucose-6-phosphatase-ß, implicated in a congenital neutropenia syndrome, is essential for macrophage energy homeostasis and functionality.
Authors:Jun HS, Cheung YY, Lee YM, Mansfield BC, Chou JY,
Journal:Blood
PubMed ID:22246029
'Glucose-6-phosphatase-ß (G6Pase-ß or G6PC3) deficiency, also known as severe congenital neutropenia syndrome 4, is characterized not only by neutropenia but also impaired neutrophil energy homeostasis and functionality. We now show the syndrome is also associated with macrophage dysfunction, with murine G6pc3(-/-) macrophages having impairments in their respiratory burst, chemotaxis, calcium ... More
Loss-of-function mutations in Rab escort protein 1 (REP-1) affect intracellular transport in fibroblasts and monocytes of choroideremia patients.
Authors:Strunnikova NV, Barb J, Sergeev YV, Thiagarajasubramanian A, Silvin C, Munson PJ, Macdonald IM,
Journal:PLoS One
PubMed ID:20027300
'BACKGROUND: Choroideremia (CHM) is a progressive X-linked retinopathy caused by mutations in the CHM gene, which encodes Rab escort protein-1 (REP-1), an escort protein involved in the prenylation of Rabs. Under-prenylation of certain Rabs, as a result of loss of function mutations in REP-1, could affect vesicular trafficking, exocytosis and ... More
Cdc42 promotes host defenses against fatal infection.
Authors:Lee K, Boyd KL, Parekh DV, Kehl-Fie TE, Baldwin HS, Brakebusch C, Skaar EP, Boothby M, Zent R,
Journal:
PubMed ID:23690402
The small Rho GTPase Cdc42 regulates key signaling pathways required for multiple cell functions, including maintenance of shape, polarity, proliferation, invasion, migration, differentiation, and morphogenesis. As the role of Cdc42-dependent signaling in fibroblasts in vivo is unknown, we attempted to specifically delete it in these cells by crossing the Cdc42(fl/fl) ... More
SOCS3 deficiency promotes M1 macrophage polarization and inflammation.
Authors:Qin H, Holdbrooks AT, Liu Y, Reynolds SL, Yanagisawa LL, Benveniste EN,
Journal:J Immunol
PubMed ID:22925925
Macrophages participate in both the amplification of inflammation at the time of injury and downregulation of the inflammatory response to avoid excess tissue damage. These divergent functions of macrophages are dictated by their microenvironment, especially cytokines, which promote a spectrum of macrophage phenotypes. The M1 proinflammatory phenotype is induced by ... More
View all Conjugués pHrodo™ BioParticles™ pour la phagocytose et kit de phagocytose, pour la cytométrie en flux citations