MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit
MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit
MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit
Applied Biosystems™

MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit

The MagMAX Pro HT NoSpin Plasmid Miniprep Kit enables scalable, ultra-rapid purification of high-quality, transfection-grade plasmid DNA (pDNA) from bacterial cultures. Using magnetic-bead technology, it simplifies workflows by eliminating centrifugation and vacuum filtration bottlenecks.
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RéférenceNbre de réactions
A5830996 réactions
A58310384 réactions
A583111 152 réactions
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Référence A58309
Prix (EUR)
362,00
Each
Nbre de réactions:
96 réactions
Prix (EUR)
362,00
Each

The Applied Biosystems MagMAX Pro HT NoSpin Plasmid Miniprep Kit provides a scalable and ultra-rapid approach for purifying high quality, transfection-grade plasmid DNA (pDNA) directly from bacterial culture. Utilizing advanced magnetic-bead technology, the MagMAX Pro HT NoSpin kit drastically simplifies and streamlines plasmid purification workflows by eliminating centrifugation and vacuum filtration bottlenecks.

MagMAX Pro HT NoSpin Plasmid Miniprep Kit features include:

  • Bead-based—utilizes dual para-magnetic beads for plasmid isolation without time-intensive processing steps (centrifugation, vacuum filtration)
  • Automation enabled—turn-key, automation-friendly protocols and scripts, allowing 96 samples to be processed in under 35 minutes on KingFisher Apex and KingFisher Flex systems
  • Fast plasmid DNA isolation—35 minutes from start to completion and ready for downstream applications
  • Wide range of compatibility—typical E. coli cultures, common medias, and range of plasmids all acceptable
  • High-quality pDNA yields of up to 20 μg, ready for downstream applications
  • Ready for transfection—isolated pDNA with very low endotoxin levels < 1 EU/μg

Specifically designed for high-throughput plasmid isolation, the MagMAX Pro HT NoSpin technology is automation friendly and suited for use with KingFisher Apex, KingFisher Flex, and KingFisher Presto purification systems, as well as integrated liquid handling robots.

The MagMAX Pro HT purification chemistry is both effective and ecofriendly. Kits include a novel endotoxin reduction buffer to remove pyrogenic contaminates, and buffers are free of ethanol and chaotropic salts.

pDNA purified with the MagMAX Pro HT NoSpin kit is suitable for a wide array of downstream applications, such as transfection, cloning, enzymatic restriction, in vitro transcription, and sequencing.

Spécifications
Volume d’élution100 μL
À utiliser avec (application)Cloning, Transfection, Protein Expression, Protein Production, mRNA production via In Vitro Transcription, Gene Editing, Sequencing, PCR, Nucleic acid labeling, PCR
À utiliser avec (équipement)KingFisher Apex, Flex and Presto Processors with 96 Deep Well Heads, Liquid Handling Robotic Systems
Nbre de réactions96 réactions
Type d’acide nucléiquePlasmid DNA (pDNA)
Compatibilité des plaquesKingFisher 96 Deep Well Plates with or without barcodes
Type de produitAutomated, pDNA extraction kit
PuretéTransfection Grade
Quantité1 Ea.
Type d’échantillonBacteria, E. coli
Volume d’échantillonBacterial Culture, 1 mL per well
BalanceMini
Distribution des tailles5-12kb
Heure du test 32 min. for 96 samples, 1 KingFisher 96 DWP
RendementUp to 20 μg pDNA using high copy plasmid
Format96 Deep-well Plate
Isolation TechnologyMagnetic Bead
Unit SizeEach
Contenu et stockage
2°C to 8°C BactBind Beads, PlasmidPure Beads, Endotoxin Reduction Buffer
15°C to 25°C RNAse A, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, Wash 1 Buffer, Wash 2 Buffer, Elution Buffer

Foire aux questions (FAQ)

Can I grow an overnight bacterial culture directly in the KingFisher 96-well plate when using the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

Yes, the KingFisher 96-well plate can be used for overnight bacterial culture growth with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit. However, growth conditions depend on factors such as media type, host strain, plasmid copy number, and plasmid size. For best results, optimize orbital shaker settings and monitor OD600 to minimize well-to-well variation and ensure consistent plasmid yield.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

How does plasmid copy number affect yields with the MagMAX™ Pro HT NoSpin Plasmid MiniPrep Kit?

High-copy plasmids yield higher amounts of DNA and are ideal for most downstream applications. Low-copy plasmids can be extracted, but yields may be lower and may not be sufficient for all transfection protocols. For low-copy plasmids, optimizing culture conditions, increasing culture volume, or performing multiple elution steps may help improve yield.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which bacterial strains are compatible with the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

The MagMAX Pro HT NoSpin Plasmid MiniPrep Kit is compatible with K12 E. coli derivatives, including DH5alpha, TOP10, and DH10B strains. Plasmid yield may vary with low-copy origins, strong host dependencies, or high genomic integration tendencies.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Should I be concerned about bead clumps in the BactBind Beads or PlasmidPure Beads bottles before use?

No, bead settling during transit is normal. This does not affect performance. We recommend vortexing well, before use.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What should I do if there are precipitates in the Lysis Buffer within the MagMAX Pro HT NoSpin Plasmid MiniPrep Kit?

We recommend warming the buffer at 30-37 degrees C for 30 min, and then inverting to mix until the precipitates dissolve.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.