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Colorant DiI (perchlorate 1,1’-dioctadécyl-3,3,3’,3’-tétraméthylindocarboxycyanine (<i>DiI</i> ; DiIC<sub>18</sub>(3)))
Citations et références (1167)
Invitrogen™

Colorant DiI (perchlorate 1,1’-dioctadécyl-3,3,3’,3’-tétraméthylindocarboxycyanine (DiI ; DiIC18(3)))

Se diffuse latéralement pour colorer l’intégralité de la cellule
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RéférenceQuantité
D391125mg, 25 mg
D282100 mg, 100 mg
Référence D3911
Prix (EUR)
437,65
Online Exclusive
554,00
Économisez 116,35 (21%)
Each
Quantité:
25mg, 25 mg
Prix (EUR)
437,65
Online Exclusive
554,00
Économisez 116,35 (21%)
Each
DiI est une coloration de membrane lipophile qui se diffuse latéralement pour colorer l’intégralité de la cellule. Sa fluorescence est faible jusqu’à ce qu’elle soit incorporée dans les membranes. Ce colorant fluorescent rouge-orangé, semblable à la tétraméthylrhodamine sur le plan spectral, est souvent utilisé comme traceur à long terme pour les cellules neuronales et autres. La coloration DiI est également disponible sous forme de solution (V-22885), sous forme de pâte (N-22880) ou sous forme solide (D-282).

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications
CouleurJaune, Jaune
Méthode de détectionFluorescence, Fluorescent
Émission565 nm
Gamme de longueur d’onde d’excitation549 nm
À utiliser avec (équipement)Microscope à fluorescence, Microscope à fluorescence
Poids moléculaire933.88
Quantité25mg, 25 mg
Conditions d’expéditionTempérature ambiante
Type de produitColoration
SubCellular LocalizationLipides & membranes cellulaires, Lipids
Unit SizeEach
Contenu et stockage
Stocker à température ambiante et à l'abri de lalumière.

Foire aux questions (FAQ)

I'm labeling live cells with Vybrant DiI or DiD lipophilic cyanine dyes. DiI gives a nice even membrane labeling, but DiD is more "spotty". What can be done?

This is expected. DiD (which is far-red fluorescent) is never as uniform as DiI (which is orange fluorescent). If uniformity is desired, try increasing the label time and concentration, but it still isn't likely to be as uniform as DiI. CellMask Deep Red Plasma Membrane stain is much more uniform and is about the same wavelength as DiD. However, if you intend to do cell tracking over days, CellMask stain has not been tried for that application.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Why do I lose all signal from my neuronal tracer when I do a methanol fixation on my cells?

If the tracer you chose is a lipophilic dye and fix with methanol, the lipids are lost with the methanol. If you have to use methanol fixation then choose a tracer that will covalently bind to proteins in the neurons.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I stained my cells with a lipophilic cyanine dye, like DiI, but the signal was lost when I tried to follow up with antibody labeling. Why?

Since these dyes insert into lipid membranes, any disruption of the membranes leads to loss of the dye. This includes permeabilization with detergents like Triton X-100 or organic solvents like methanol. Permeabilization is necessary for intracellular antibody labeling, leading to loss of the dye. Instead, a reactive dye such as CFDA SE should be used to allow for covalent attachment to cellular components, thus providing for better retention upon fixation and permeabilization.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I labeled my neurons with DiI and then fixed and permeabilized and now I have no signal. What did I do wrong?

DiI is a lipophilic dye that resides mostly in lipids in the cell, when cells are permeabilized with detergent or fixed using alcohol this strips away the lipid and the dye. If permeabilization is required CM-DiI can be used because this binds covalently to proteins in the membrane; some signal is lost upon fixation/permeabilization, but enough signal should be retained to make detection possible.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How long does it take for lipophlic tracers to transport along the membrane? How much faster are the FAST lipophilic dyes?

The transport is fairly slow, around 6 mm/day in live tissue and slower in fixed tissue, so diffusion of lipophilic carbocyanine tracers from the point of their application to the terminus of a neuron can take several days to weeks The FAST DiO and DiI analogs (which have unsaturated alkyl tails) can improve transport rate by around 50%.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

View all Colorant DiI (perchlorate 1,1’-dioctadécyl-3,3,3’,3’-tétraméthylindocarboxycyanine (DiI ; DiIC18(3))) FAQs

Citations et références (1167)

Citations et références
Abstract
Using fluorescent dyes for fate mapping, lineage analysis, and axon tracing in the chick embryo.
Authors:Clarke JD
Journal:Methods in molecular biology (Clifton, N.J.)
PubMed ID:19030810
Angiotensin II induces LOX-1, the human endothelial receptor for oxidized low-density lipoprotein.
Authors:Morawietz H,Rueckschloss U,Niemann B,Duerrschmidt N,Galle J,Hakim K,Zerkowski HR,Sawamura T,Holtz J
Journal:Circulation
PubMed ID:10468518
Neural transplant staining with DiI and vital imaging by 2-photon laser-scanning microscopy.
Authors:Potter SM, Pine J, Fraser SE
Journal:Scanning Microsc Suppl
PubMed ID:9601539
We are developing a multielectrode silicon "neuroprobe" for maintaining a long-term, specific, two-way electrical interface with nervous tissue. Our approach involves trapping a neuron (from an embryonic rat hippocampus) in a small well with a stimulation/recording electrode at its base. The well is covered with a grillwork through which the ... More
Cultured postnatal rat septohippocampal neurons change intracellular calcium in response to ethanol and nerve growth factor.
Authors:Webb B, Suarez SS, Heaton MB, Walker DW
Journal:Brain Res
PubMed ID:9459553
Ethanol exposure affects cellular mechanisms involved in the regulation of calcium (Ca2+) homeostasis. Neurotrophins, such as nerve growth factor (NGF), stabilize intracellular Ca2+([Ca2+]i) during a variety of neurotoxic insults. In this study, changes in [Ca2+]i during treatment with ethanol and NGF were measured at the cell body of neurons using ... More
Evaluation of a flow cytometric fluorescence quenching assay of phagocytosis of sensitized sheep erythrocytes by polymorphonuclear leukocytes.
Authors:Van Amersfoort ES, Van Strijp JA
Journal:Cytometry
PubMed ID:7875036
A number of reports have been published describing phagocytosis assays for flow cytometric analysis. In some of these, the fluorescence quenching technique has been used to discriminate between adherent and ingested particles. In this report, we have evaluated the efficacy of a quantitative fluorescence quenching technique with crystal violet and ... More
View all Colorant DiI (perchlorate 1,1’-dioctadécyl-3,3,3’,3’-tétraméthylindocarboxycyanine (DiI ; DiIC18(3))) citations