Citations et références (5)

Invitrogen™

Pro-Q™ Diamond Phosphoprotein Blot Stain Kit

Le Kit de coloration de transfert de phosphoprotéines Diamond Pro-Q offre une méthode rapide et simple pour détecter directement lesAfficher plus

Référence	Quantité
P33356	1 kit

Référence P33356

Prix (EUR)

596,00

Quantité:

1 kit

Grand volume ou format personnalisé

Prix (EUR)

596,00

Le Kit de coloration de transfert de phosphoprotéines Diamond Pro-Q offre une méthode rapide et simple pour détecter directement les phosphoprotéines sur les membranes en difluorure de polyvinylidène (PVDF) ou en nitrate de cellulose. Le colorant fluorescent se lie sélectivement à la fraction phosphate et la liaison est indépendante du contexte de séquence d’acide aminé. Parce qu’il s’agit d’une coloration directe, vous n’avez pas besoin d’anticorps ou de radio-isotopes coûteux. Le kit de coloration de transfert de phosphoprotéines Diamond Pro-Q peut être utilisé pour analyser l’état de phosphorylation natif des protéines obtenues à partir d’échantillons de tissus ou de liquides organiques.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Emplacement de détectionDétection sur transfert

Méthode de détectionFluorescent

Quantité1 kit

Durée de conservation6 mois

Molécule cibleProtéines (phosphoprotéines)

Marqueur ou colorantPro-Q Diamond

Gamme de produitsPRO-Q

Type de produitKit de coloration de transfert phosphoprotéines

Unit SizeEach

Citations et références (5)

Citations et références

Abstract

The human mitochondrial transcription termination factor (mTERF) is fully active in vitro in the non-phosphorylated form.

Authors:Asin-Cayuela J, Schwend T, Farge G, Gustafsson CM,

Journal:J Biol Chem

PubMed ID:15899902

'The human mitochondrial transcription termination factor (mTERF) is a 39-kDa protein that terminates transcription at the 3''-end of the 16 S rRNA gene and thereby controls expression of the ribosomal transcription unit of mitochondrial DNA. The transcription termination activity of human mTERF has been notoriously difficult to study in vitro, ... More

Ca(2+)-related signaling and protein phosphorylation abnormalities play central roles in a new experimental model of electrical storm.

Authors:Tsuji Y, Hojo M, Voigt N, El-Armouche A, Inden Y, Murohara T, Dobrev D, Nattel S, Kodama I, Kamiya K,

Journal:Circulation

PubMed ID:21555709

Electrical storm (ES), characterized by recurrent ventricular tachycardia/fibrillation, typically occurs in implantable cardioverter-defibrillator patients and adversely affects prognosis. However, the underlying molecular basis is poorly understood. In the present study, we report a new experimental model featuring repetitive episodes of implantable cardioverter-defibrillator firing for recurrent ventricular fibrillation (VF), in which ... More

Pin1 promotes histone H1 dephosphorylation and stabilizes its binding to chromatin.

Authors:Raghuram N, Strickfaden H, McDonald D, Williams K, Fang H, Mizzen C, Hayes JJ, Th'ng J, Hendzel MJ,

Journal:

PubMed ID:24100296

Histone H1 plays a crucial role in stabilizing higher order chromatin structure. Transcriptional activation, DNA replication, and chromosome condensation all require changes in chromatin structure and are correlated with the phosphorylation of histone H1. In this study, we describe a novel interaction between Pin1, a phosphorylation-specific prolyl isomerase, and phosphorylated ... More

Quantitative analysis of protein phosphorylation status and protein kinase activity on microarrays using a novel fluorescent phosphorylation sensor dye.

Authors:Martin K, Steinberg TH, Cooley LA, Gee KR, Beechem JM, Patton WF

Journal:Proteomics

PubMed ID:12872225

Ultrasensitive detection of minute amounts of phosphorylated proteins and peptides is a key requirement for unraveling many of the most important signal transduction pathways in mammalian systems. Protein microarrays are potentially useful tools for sensitive screening of global protein expression and post-translational modifications, such as phosphorylation. However, the analysis of ... More

Detection of phosphoproteins on electroblot membranes using a small-molecule organic fluorophore.

Authors:Goodman T, Schulenberg B, Steinberg TH, Patton WF

Journal:Electrophoresis

PubMed ID:15300773

A new formulation of the small-molecule organic fluorophore, Pro-Q Diamond dye, has been developed that permits rapid and simple detection of phosphoproteins directly on polyvinylidene difluoride (PVDF) or nitrocellulose membranes (electroblots). Protein samples are first separated by electrophoresis and then electroblotted to membranes, stained and destained, in an analogous manner ... More