SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells

Citations et références (14)

Invitrogen™

SelectFX™ Nuclear Labeling Kit (DAPI, SYTOX™ Green, 7-AAD, TO-PRO™-3 Iodide), for fixed cells

Le kit de marquage SelectFX™ Nuclear fournit quatre colorants fluorescents distincts sur le plan spectral : DAPI bleu fluorescent, SYTOX™Afficher plus

Référence	Quantité
S33025	1 kit

Référence S33025

Prix (EUR)

321,00

Quantité:

1 kit

Prix (EUR)

321,00

Le kit de marquage SelectFX™ Nuclear fournit quatre colorants fluorescents distincts sur le plan spectral : DAPI bleu fluorescent, SYTOX™ vert florescent, 7-aminoactinomycine D rouge fluorescent (7-AAD) et colorant rouge lointain fluorescent TO-PRO™-3. Ces colorants sont parfaits pour être utilisés comme des contre-colorants dans les applications multicolores ; il suffit de sélectionner la coloration qui contraste de façon spectrale avec d’autres sondes fluorescentes appliquées sur l’échantillon. Lorsqu’ils sont utilisés conformément au protocole fourni, les colorants du kit de marquage SelectFX™ Nuclear offrent une coloration nucléaire extrêmement sélective avec peu ou pas de marquage cytoplasmique.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

CouleurVert, Vert

Méthode de détectionFluorescence, Fluorescent

Type de colorantImperméant aux cellules

À utiliser avec (équipement)Microscope à fluorescence, cytomètre en flux, Microscope à fluorescence, cytomètre en flux

Gamme de produitsSELECTFX, SYTOX, TO-PRO

Quantité1 kit

Conditions d’expéditionTempérature ambiante

Type d’étiquetteFluorescent Dye

Type de produitKit de marquage nucléaire

SubCellular LocalizationNoyau, acides nucléiques, Nucleus

Unit SizeEach

Contenu et stockage

Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this?

Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

I am using SYTOX AAdvanced as a dead cell stain, but all of my cells are labeling even though I am certain that they are supposed to be alive. These are adherent cells that I have trypsinized. Why am I getting false-dead signals?

SYTOX AAdvanced labels only dead cells because it is a cell impermeant dye. The dye can only enter cells that have a compromised plasma membrane. Trypsinization may cause temporary disruption of the plasma membrane, sufficient to allow staining with a cell impermeant dye. You can reduce the “false-dead” problem by either reducing the amount of trypsin and/or reduce the incubation time for trypsinization or use a gentler dissociation reagent such as TrypLE Express, TrypLESelect reagents, or Versene. After trypsinization, wash well, and if possible, allow a recovery time in normal culture media before staining with any of the SYTOX dyes.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (14)

Citations et références

Abstract

Avoiding cytotoxicity of transposases by dose-controlled mRNA delivery.

Authors:Galla M, Schambach A, Falk CS, Maetzig T, Kuehle J, Lange K, Zychlinski D, Heinz N, Brugman MH, Göhring G, Izsvák Z, Ivics Z, Baum C,

Journal:Nucleic Acids Res

PubMed ID:21609958

'The Sleeping Beauty (SB) transposase and its newly developed hyperactive variant, SB100X, are of increasing interest for genome modification in experimental models and gene therapy. The potential cytotoxicity of transposases requires careful assessment, considering that residual integration events of transposase expression vectors delivered by physicochemical transfection or episomal retroviral vectors ... More

Protein transduction from retroviral Gag precursors.

Authors:Voelkel C, Galla M, Maetzig T, Warlich E, Kuehle J, Zychlinski D, Bode J, Cantz T, Schambach A, Baum C,

Journal:Proc Natl Acad Sci U S A

PubMed ID:20385817

Retroviral particles assemble a few thousand units of the Gag polyproteins. Proteolytic cleavage mediated by the retroviral protease forms the bioactive retroviral protein subunits before cell entry. We hypothesized that this process could be exploited for targeted, transient, and dose-controlled transduction of nonretroviral proteins into cultured cells. We demonstrate that ... More

Insulin-like growth factor-1 sustains stem cell mediated renal repair.

Authors:Imberti B, Morigi M, Tomasoni S, Rota C, Corna D, Longaretti L, Rottoli D, Valsecchi F, Benigni A, Wang J, Abbate M, Zoja C, Remuzzi G,

Journal:J Am Soc Nephrol

PubMed ID:17942965

In mice with cisplatin-induced acute kidney injury, administration of bone marrow-derived mesenchymal stem cells (MSC) restores renal tubular structure and improves renal function, but the underlying mechanism is unclear. Here, we examined the process of kidney cell repair in co-culture experiments with MSC and cisplatin-injured proximal tubular epithelial cells (PTEC). ... More

Dual probe with fluorescent and magnetic properties for imaging solid tumor xenografts.

Authors:Shan L, Wang S, Sridhar R, Bhujwalla ZM, Wang PC

Journal:Mol Imaging

PubMed ID:17445503

A dual probe with fluorescent and magnetic reporter groups was constructed by linkage of the near-infrared (NIR) fluorescent transferrin conjugate (Tf(NIR)) on the surface of contrast agent-encapsulated cationic liposome (Lip-CA). This probe was used for magnetic resonance imaging (MRI) and optical imaging of MDA-MB-231-luc breast cancer cells grown as a ... More

Using total internal reflection fluorescence microscopy to visualize rhodopsin-containing cells.

Authors:

Journal:Appl Environ Microbiol

PubMed ID:25769822

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