Tubuline Tracker™ Green (Oregon Green™ 488 Taxol, bis-acétate), pour l’imagerie en cellules vivantes

Citations et références (30)

Invitrogen™

Tubuline Tracker™ Green (Oregon Green™ 488 Taxol, bis-acétate), pour l’imagerie en cellules vivantes

Le Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acétate) assure la coloration vert fluorescent de la tubuline polymérisée dans lesAfficher plus

Référence	Quantité
T34075	1 jeu

Référence T34075

Prix (EUR)

757,65

Online Exclusive

824,00

Économisez 66,35 (8%)

1 set

Quantité:

1 jeu

Prix (EUR)

757,65

Online Exclusive

824,00

Économisez 66,35 (8%)

1 set

Le Tubulin Tracker™ Green (Oregon Green™ 488 Taxol, bis-acétate) assure la coloration vert fluorescent de la tubuline polymérisée dans les cellules vivantes. Le Tubulin Tracker™ Green est un composé non fluorescent et non chargé qui traverse facilement la membrane plasmique des cellules vivantes. Une fois à l’intérieur de la cellule, le groupe de blocage lipophile est clivé par des estérases non spécifiques, ce qui crée une forme chargée à fluorescence verte.

Usage exclusivement réservé à la recherche. Ne pas utiliser pour des procédures de diagnostic.

Spécifications

Type de colorantOregon Green™ 488

Gamme de produitsOregon Green

Quantité1 jeu

Conditions d’expéditionTempérature ambiante

Type d’étiquetteColorants classiques

Type de produitTraqueur de tubuline

Sub Cellular LocalizationTubuline, cytosquelette, Tubulin

Unit Size1 set

Contenu et stockage

Stocker au congélateur (entre -5°C et -30°C) à l’abri de la lumière.

Foire aux questions (FAQ)

What is the difference between Tubulin Tracker Green (Cat No. T34078) and Tubulin Tracker Green, for live cell imaging (Cat. No. T34075)?

Both products include the same Tubulin Tracker Green reagent. The difference is in the packaging and amount of Tubulin Tracker Green reagent contained. Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) includes enough Tubulin Tracker Green reagent for 300 slides whereas Tubulin Tracker Green (Cat No. T34078) contains enough Tubulin Tracker Green reagent for 60 slides. Also, Tubulin Tracker Green, for live cell imaging (Cat. No. T34075) does not include Probenecid.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

With Tubulin Tracker Deep Red and Tubulin Tracker Green, can I fix samples after labeling?

No. The labeling is not retained upon fixation. Imaging of the samples should be done only on live cells. To limit photobleaching while viewing live cells, use ProLong Live Antifade Reagent, for live cell imaging (Cat No. P36974 and P36975).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is Docetaxel in Tubulin Tracker Deep Red different from Taxol in Tubulin Tracker Green? Are there any differences in the binding or cytotoxicity between the two reagents?

Docetaxel has a slightly higher binding affinity than Taxol and exhibits longer retention time due to better uptake and slower efflux, but for use in labeling cultured cells, these differences should be minimal. More information comparing the properties of these two reagents is provided in this publication: https://www.ncbi.nlm.nih.gov/pubmed/1671606 https://www.cell.com/fulltext/S1074-5521(05)00301-7

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations et références (30)

Citations et références

Abstract

PKC-induced intracellular trafficking of Ca(V)2 precedes its rapid recruitment to the plasma membrane.

Authors:Zhang Y, Helm JS, Senatore A, Spafford JD, Kaczmarek LK, Jonas EA,

Journal:J Neurosci

PubMed ID:18322103

'Activation of protein kinase C (PKC) potentiates secretion in Aplysia peptidergic neurons, in part by inducing new sites for peptide release at growth cone terminals. The mechanisms by which ion channels are trafficked to such sites are, however, not well understood. We now show that PKC activation rapidly recruits new ... More

Modulation of hippocampal calcium signalling and plasticity by serine/threonine protein phosphatases.

Authors:Koss DJ, Hindley KP, Riedel G, Platt B

Journal:J Neurochem

PubMed ID:17442047

'Kinases and phosphatases act antagonistically to maintain physiological phosphorylation/dephosphorylation at numerous intracellular sites critical for neuronal signalling. In this study, it was found that inhibition of serine/threonine phosphatases by exposure of hippocampal slices to okadaic acid (OA) or cantharidin (CA; 100 nmol/L) for 2 h resulted in reduced basal synaptic ... More

Redundant mechanisms recruit actin into the contractile ring in silkworm spermatocytes.

Authors:Chen W, Foss M, Tseng KF, Zhang D,

Journal:PLoS Biol

PubMed ID:18767903

'Cytokinesis is powered by the contraction of actomyosin filaments within the newly assembled contractile ring. Microtubules are a spindle component that is essential for the induction of cytokinesis. This induction could use central spindle and/or astral microtubules to stimulate cortical contraction around the spindle equator (equatorial stimulation). Alternatively, or in ... More

2-(Naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone inhibits skin cancer M21 cell proliferation through aberrant expression of microtubules and the cell cycle.

Authors:Wu YC, Hour MJ, Leung WC, Wu CY, Liu WZ, Chang YH, Lee HZ,

Journal:J Pharmacol Exp Ther

PubMed ID:21652781

'Microtubules are a proven target for anticancer drug development because they are critical for mitotic spindle formation and the separation of chromosomes at mitosis. 2-(Naphthalene-1-yl)-6-pyrrolidinyl-4-quinazolinone (HL66) induced cell death with the large cells and multiple micronuclei in M21 skin cancer cells. We demonstrated that HL66-induced cell death is caspase-independent and ... More

Metalloprotease meprin beta generates nontoxic N-terminal amyloid precursor protein fragments in vivo.

Authors:Jefferson T, Cauševic M, auf dem Keller U, Schilling O, Isbert S, Geyer R, Maier W, Tschickardt S, Jumpertz T, Weggen S, Bond JS, Overall CM, Pietrzik CU, Becker-Pauly C,

Journal:J Biol Chem

PubMed ID:21646356

'Identification of physiologically relevant substrates is still the most challenging part in protease research for understanding the biological activity of these enzymes. The zinc-dependent metalloprotease meprin ß is known to be expressed in many tissues with functions in health and disease. Here, we demonstrate unique interactions between meprin ß and ... More

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