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Citations & References (1)
Invitrogen™

Membrane Blocking Solution

Membrane Blocking Solution
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Catalog NumberQuantity
0001051 L
Catalog number 000105
Price (HKD)
2,164.00
Each
Quantity:
1 L
Price (HKD)
2,164.00
Each
Membrane Blocking Solution can be used to block nitrocellulose and PVDF membranes, as well as to dilute antibodies or enzyme conjugates. This solution is a Tris buffer containing bovine serum albumin, goat IgG, Tween 20, and a mixture of other components that can reduce non-specific binding. The Proclin used in this reagent does not inhibit peroxidase or alkaline phosphatase activity. Note that this blocking solution contains normal goat IgG and should not be used in assays where anti-goat immunoglobulins are employed.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Name or MaterialMembrane Blocking Solution
Recommended StorageStore this reagent at 2-8°C. DO NOT FREEZE. If precipitation occurs, filter to clarify before use.
Quantity1 L
Unit SizeEach

Frequently asked questions (FAQs)

How can I reduce background bands in my Western blot?

Optimize the concentration of primary and secondary antibodies. In some cases, increasing the concentration of blocking agent (BSA or non-fat dry milk) or usiing an alternative blocking solution such as Starting Block or SuperBlock may reduce background signal. After incubation with the primary antibody, wash at least 2 times with TBST (include 0.5 M NaCl in one or more of the wash steps). Avoid Nonidet P40 or Triton X-100 in buffers because protein detection is decreased when these detergents are used.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

Citations & References (1)

Citations & References
Abstract
Serine phosphorylation of p60 tumor necrosis factor receptor by PKC-delta in TNF-alpha-activated neutrophils.
Authors:Kilpatrick LE, Song YH, Rossi MW, Korchak HM,
Journal:Am J Physiol Cell Physiol
PubMed ID:11078718
Tumor necrosis factor-alpha (TNF-alpha) triggers degranulation and oxygen radical release in adherent neutrophils. The p60TNF receptor (p60TNFR) is responsible for proinflammatory signaling, and protein kinase C (PKC) is a candidate for the regulation of p60TNFR. Both TNF-alpha and the PKC-activator phorbol 12-myristate 13-acetate triggered phosphorylation of p60TNFR. Receptor phosphorylation was ... More