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Invitrogen™
CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need forRead more
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| Catalog Number | No. of Reactions |
|---|---|
| 11754100 | 100 Reactions |
| 11754500 | 500 Reactions |
Catalog number 11754100
Price (HKD)
8,699.00
Each
No. of Reactions:
100 Reactions
Price (HKD)
8,699.00
Each
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Features of the CellsDirect™ One-Step qRT-PCR Kit include:
• Efficiency—Offers significant time and cost savings for increased throughput
• Convenience—Enzyme mix includes both SuperScript™ III Reverse Transcriptase and Platinum™ Taq DNA Polymerase
• Sensitivity—No separate RNA isolation step increases sensitivity by minimizing sample loss
• Broad Detection Range—Detect targets from samples ranging from a single cell to 10,000 cells
• Versatility—Compatible with a wide variety of cell lines and samples collected by Laser Capture Microdissection (LCM)
Streamline Your Workflow
In traditional real-time quantitative RT-PCR (qRT-PCR), you first isolate RNA from cells in a time-consuming procedure that can lead to loss of material. Using the CellsDirect™ One-Step qRT-PCR Kit, you lyse the cells and add the complete lysate directly to a one-step qRT-PCR reaction with minimal handling and sample loss.
Ideal for Your qRT-PCR Application
CellsDirect™ technology is validated for gene expression studies involving limited samples, and is suitable for validation of RNAi knockdowns. CellsDirect™ One-Step qRT-PCR Kits are especially valuable for high-throughput applications by eliminating costly, time-consuming RNA purification steps (Figure 1). The CellsDirect™ One-Step qRT-PCR Kit may also be used to detect genomic DNA targets.
CellsDirect™ One-Step qRT-PCR Kits may be used in conjunction with hydrolysis probes (e.g. TaqMan™) or LUX™ fluorogenic primers.
Note: This kit includes 2X Reaction Mix containing ROX™ Reference Dye. An equivalent kit (11753-500), containing a separate tube of ROX™ Reference Dye, is also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Equipment)7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system
No. of Reactions100 Reactions
Product LineCellsDirect, Platinum, SuperScript
Quantity100 reactions
Sample TypeCells
Concentration2X
Detection MethodPrimer-probe
For Use With (Application)RT-PCR
Reaction SpeedFast or Standard
Unit SizeEach
Contents & Storage
• Resuspension Buffer, 10 mL
• Lysis Enhancer, 1 mL
• 1 U/μL DNase I, Amplification Grade, 500 μL
• 10X DNase I Buffer, 160 μL
• 25 mM EDTA, 400 μL
• SuperScript™ III RT/Platinum™ Taq Mix, 100 μL
• 2X Reaction Mix with ROX™, 2 x 1.25 mL
• 50 mM MgSO4, 1 mL
• DEPC-treated water, 2 mL
• 10 ng/μL HeLa Total RNA, 10 μL
The kit is shipped on dry ice and should be stored at -20°C.
• Lysis Enhancer, 1 mL
• 1 U/μL DNase I, Amplification Grade, 500 μL
• 10X DNase I Buffer, 160 μL
• 25 mM EDTA, 400 μL
• SuperScript™ III RT/Platinum™ Taq Mix, 100 μL
• 2X Reaction Mix with ROX™, 2 x 1.25 mL
• 50 mM MgSO4, 1 mL
• DEPC-treated water, 2 mL
• 10 ng/μL HeLa Total RNA, 10 μL
The kit is shipped on dry ice and should be stored at -20°C.