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Citations & References (3)
Invitrogen™
Gateway™ Vector Conversion System with One Shot™ ccdB Survival Cells
The Gateway™ Vector Conversion System is designed to convert any vector into a Gateway™ destination vector using restriction endonucleases andRead more
| Catalog Number | Quantity |
|---|---|
| 11828029 | 1 kit |
Catalog number 11828029
Price (HKD)
7,608.00
Each
Quantity:
1 kit
Price (HKD)
7,608.00
Each
The Gateway™ Vector Conversion System is designed to convert any vector into a Gateway™ destination vector using restriction endonucleases and ligase. A Gateway™ cassette containing attR recombination sites flanking a ccdB gene (1) and a chloramphenicol-resistance gene are blunt-end cloned into the multiple cloning site of any vector. ccdB Survival 2 competent cells allow propagation of the Gateway™ vectors containing the ccdB gene. The Gateway™ Vector Conversion System offers:
• Conversion of any expression vector into a Gateway™ destination vector
• Three cassettes to construct a destination vector in the correct reading frame. Each cassette has a unique restriction site to easily distinguish the cassettes and screen for the correct orientation.
• Conversion of any expression vector into a Gateway™ destination vector
• Three cassettes to construct a destination vector in the correct reading frame. Each cassette has a unique restriction site to easily distinguish the cassettes and screen for the correct orientation.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Antibiotic Resistance BacterialChloramphenicol (CmR)
Bacterial or Yeast StrainccdB Survival™ 2
Product TypeVector Conversion System
Quantity1 kit
Cloning MethodGateway
Product LineOne Shot
Unit SizeEach
Contents & Storage
The Gateway™ Vector Conversion System contains Gateway™ Reading Frame Cassettes A, B, and C.1, pENTR™-gus positive control, and One Shot™ ccdB Survival Cells. Store DNA at -20°C and competent cells at -80°C.
Citations & References (3)
Citations & References
Abstract
A modified Gateway cloning strategy for overexpressing tagged proteins in plants.
Journal:Plant Methods
PubMed ID:18211686
'ABSTRACT: BACKGROUND: Recent developments, including the sequencing of a number of plant genomes, have greatly increased the amount of data available to scientists and has enabled high throughput investigations where many genes are investigated simultaneously. To perform these studies, recombinational cloning methods such as the Gateway system have been adapted
Trpm7 regulates cell adhesion by controlling the calcium dependent protease calpain.
Journal:J Biol Chem
PubMed ID:16436382
'M-calpain is a protease implicated in the control of cell adhesion through focal adhesion disassembly. The mechanism by which the enzyme is spatially and temporally controlled is not well understood, particularly because calpain''s dependence on calcium exceeds the sub-micromolar concentrations normally observed in cells. Here we show that the channel-kinase
High-throughput gateway bicistronic retroviral vectors for stable expression in mammalian cells: exploring the biologic effects of STAT5 overexpression.
Journal:DNA Cell Biol
PubMed ID:15231069
Stable expression of cloned genes in mammalian cells has been achieved in the past by retroviral transduction using bicistronic retroviral vectors. In these vectors, the use of an Internal Ribosome Entry Site (IRES) allows simultaneous expression of a protein of interest and a fluorescence marker. However, traditional cDNA cloning in