The Thermo Scientific pCMV-Green Renilla Luc vector is a derivative of pMCS-Green Renilla Luc. It contains the luciferase gene under the control of the Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, creating a constitutive expression vector that can be used as a normalization control to account for experimental variation in combination with other reporters.
Features of the pTK-Green Renilla Luc vector:
• Weak (TK) constitutive promoter for co-transfection and normalization • Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively • High-copy pUC bacterial DNA replication origin