KingFisher™ Flex Purification System

Name: KingFisher™ Flex Purification System
SKU: 24074441

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Catalog Number	Product Type
24074441	24 PCR head and heating block
24074410	96 PCR Head
24074411	96 PCR head and heating block
24074431	96 DW head and heating block
24074420	96 DW Head
24074421	96 KF head and heating block
24075410	96 PCR heating block
5400620	96 PCR Plate
A39952	96 Plate and Warranty
A39966	96 Plate with SmartStart

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Catalog number 24074441

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Product Type:

24 PCR head and heating block

Prepare samples efficiently from a variety of materials with Thermo Scientific KingFisher Flex Purification Systems, which offer highly versatile, automated magnetic-particle processing for DNA/RNA, protein or cell purification from virtually any source. Using revolutionary magnetic particle separation technology, this system provides excellent reproduciblity and quality.

Find a flexible solution for different types of sample processing needs. With 24 configurations, customers can raise the processing volume up to 5 mL, resulting in increased yield of the purified product. For higher throughput needs, 96 samples can be processed with 96 magnet head.

Fully automated system yields high-speed purification of nucleic acids, proteins and cells.
High throughput system processes up to 96 samples in less than 20 minutes.
Expands sample volume by 10-fold with the 24-well format.
Open and flexible system lets the customer use anymagnetic particle-based kit tomeet the application demands.
Easy-to-use Thermo Scientific™ BindIt™ Software provides instrument control, protocol creation andmodification.
Ready-made protocols for different type of applications are available.

Recommended for:

Genomics and proteomics
Target identification
Veterinary assays
Biomarker discovery
Quality control
Forensics

For Laboratory Use

Specifications

For Use With (Application)Genomics and proteomics, target identification, veterinary assays, biomarker discovery, quality control, forensics

Run Time20 min.

Product Type24 PCR head and heating block

Unit SizeEach

Frequently asked questions (FAQs)

How can I calibrate the heating block for the KingFisher Flex instrument if it is off by 1 degree Celsius or more?

The accuracy of the KingFisher Flex heating block is ± 1°C for up to 80°C, and ± 2°C for up to 115°C (see page 72 of the manual). If there is an issue with the accuracy of the heating block, you can contact to schedule a service call. Calibration of the heating block can only be performed by one of our engineers.

Find additional tips, troubleshooting help, and resources within our Automated Nucleic Acid Purification Support Center.

Which plastic consumables can I use with the KingFisher Flex 24 Deep-Well Head?

A list of necessary plastic consumables for the KingFisher Flex 24 Deep-Well Head can be found on the KingFisher Plastics for 24 deep-well format product page.

Are the magnets on the KingFisher Flex Purification System magnetic head strong enough to disturb nearby laboratory equipment?

We do not recommend placing the KingFisher Flex Purification System in close proximity to magnetic tapes, computer discs or other magnetic storage systems, such as credit cards, as these can be damaged by the strong magnetic field of the KingFisher Flex magnetic heads. Do not hold the KingFisher Flex magnetic heads close to a PC display, since this may cause damage to the display. Do not use metal tools when handling KingFisher Flex magnetic heads. People wearing a pacemaker or metallic prostheses should also not use this product as these items could be affected or damaged by close contact with a strong magnetic field.

How does the KingFisher Flex Purification heating block work?

The KingFisher Flex Purification System heating block is located inside the instrument and can be used automatically during the protocol. All KingFisher Flex plates can be heated using specially designed, interchangeable heating blocks. Any number of heating steps can be added to a protocol. When a protocol enters a heating step, the plate is automatically moved to the dedicated heating position for heating. Afterwards, the protocol continues automatically to the next step.

Can I use magnetic beads for protein isolation with the KingFisher Flex Purification System? If so, do you recommend any specific magnetic beads and are KingFisher Flex script protocol (.bdz) files available for these applications?

Any kind of protein purification can be done on the KingFisher Flex or Duo Prime as long as it is based on magnetic beads. Some template protocols for different magnetic beads are available for the KingFisher Flex and Duo Prime at this website: https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/automated-purification-extraction/automated-protocols-software.html?open=protein.
Please know that these templates are only a starting point and each protein and application is unique so optimization will be required. This optimization and protocol modification is the responsibility of the end user.

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