Dynabeads™ MyOne™ Silane
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Invitrogen™

Dynabeads™ MyOne™ Silane

An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simpleRead more
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Catalog NumberQuantity
37002D5 mL
Catalog number 37002D
Price (HKD)
3,799.00
Each
Add to cart
Quantity:
5 mL
Price (HKD)
3,799.00
Each
Add to cart
An excellent tool for highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. Please note that this is a stand-alone product containing only Dynabeads™ in a storage buffer. Our products Dynabeads™ SILANE genomic DNA (Cat.no. 370-12D) or Dynabeads™ SILANE viral NA (Cat.no. 370-11D) include the appropriate buffers and protocols you need to extract genomic DNA or viral nucleic acids respectively.

Magnetic particles from alternative suppliers often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. With Dynabeads™ MyOne™ SILANE you are ensured a higher sensitivity, capacity and performance.

The small 1 μm Dynabeads™ MyOne™ SILANE have an optimized silica-like surface chemistry and a high specific surface area, providing efficient kinetics and a high sensitivity in nucleic acid capture. Their uniform size and surface area also ensure reproducible results. The product holds reputable Dynal™ high standards with respect to within- and between-lot reproducibility and automation ability.

Advantages:

• Highly predictable binding per mg of beads
• High sensitivity, allowing for a low detection limit
• For isolation of viral DNA⁄RNA, total RNA, genomic DNA, etc.
• Isolates both DNA and RNA
• Quicker than spin columns
• More cost-effective, yet performs to the same level as spin columns
• Automation-friendly (slow sedimentation rate + high magnetic mobility)
• Adjusted to customer requirements on a custom OEM basis

Applications:
• Isolation of total nucleic acids (DNA⁄RNA)from typical biological samples (e.g. serum⁄plasma, whole blood, etc.)
• Well suited for automated assays.
• Dedicated kits have been developed for isolation of viral nucleic acids and genomic DNA (available separately).

Additional Info:
Validation and customization (bead, buffer, protocol or format) can be made available on a custom OEM basis. For further information and price quotes for larger volumes of the silica-like Dynabeads™ MyOne™ SILANE, please contact us at: ivd@invitrogen.com.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration40 mg/mL
Product LineDYNAL, Dynabeads, MyOne
Quantity5 mL
Shipping ConditionRoom Temperature
Surface FunctionalitySilanol (Silica-Like)
Diameter (Metric)1 μm
Product TypeSILANE
Unit SizeEach
Contents & Storage
Supplied at a concentration of 40 mg/mL. 5 mL total volume, sufficient for 96 isolations.

Store at: 2°C to 8°C.

Frequently asked questions (FAQs)

My Dynabeads magnetic beads are not pelleting well with the magnet. Do you have any suggestions for me?

Please review the following possibilities for why your Dynabeads magnetic beads are not pelleting:

- The solution is too viscous.
- The beads have formed aggregates because of protein-protein interaction.

Try these suggestions: - Increase separation time (leave tub on magnet for 2-5 minutes)
- Add DNase I to the lysate (~0.01 mg/mL)
- Increase the Tween 20 concentration to ~0.05% of the binding and/or washing buffer.
- Add up to 20 mM beta-merecaptoethanol to the binding and/or wash buffers.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

Can Dynabeads SILANE beads be used for nucleosome isolation?

That depends. If you want to isolate intact nucleosomes (histones + DNA complex), that is not possible with Dynabeads SILANE beads. But you can isolate DNA from nucleosomes if they are not cross-linked. For chromatin immunoprecipitation, you could consider the MAGnify Chromatin Immunoprecipitation System.

How much DNA is recovered per isolation using the Dynabeads DNA DIRECT Universal Kit?

The yield will depend on the number of nucleated cells present in the sample. One unit (200 µL) of Dynabeads DNA DIRECT Universal Kit will isolate at least 200 ng of high quality, PCR-ready genomic DNA per sample, sufficient for 10 PCR reactions. The size of the sample used should be adjusted to match the Dynabeads magnetic beads capacity. A typical starting sample is 10,000 cultured cells or 10 µL blood. The protocol can be adjusted to support 30 µL blood samples, sufficient for 30-50 downstream PCR amplifications (600 ng to 1 µg DNA).

I have a long double-stranded DNA fragment I would like to isolate. What product do you recommend?

For biotin-labeled DNA that is less than 1 kb, we recommend you use Dynabeads M270 Streptavidin (Cat. No. 65305) and MyOne C1 magnetic beads (Cat. No. 65001). We recommend our Dynabeads KilobaseBINDER Kit (Cat. No. 60101), which is designed to immobilize long (>1 kb) double-stranded DNA molecules. The KilobaseBINDER reagent consists of M-280 Streptavidin-coupled Dynabeads magnetic beads along with a patented immobilization activator in the binding solution to bind to long, biotinylated DNA molecules for isolation. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/immobilisation-of-long-biotinylated-dna-fragments.html) for more information in regards to long biotinylated DNA fragment isolation.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.

Can I use Dynabeads magnetic beads to isolate single-stranded DNA templates?

Yes, Dynabeads magnetic beads can be used to isolate single-stranded DNA. Streptavidin Dynabeads magnetic beads can be used to target biotinylated DNA fragments, followed by denaturation of the double-stranded DNA and removal of the non-biotinylated strand. The streptavidin-coupled Dynabeads magnetic beads will not inhibit any enzymatic activity. This enables further handling and manipulation of the bead-bound DNA directly on the solid phase. Please see the following link (https://www.thermofisher.com/us/en/home/life-science/dna-rna-purification-analysis/napamisc/capture-of-biotinylated-targets/preparing-single-stranded-dna-templates.html) for more information in regards to single-stranded DNA capture.

Find additional tips, troubleshooting help, and resources within our Dynabeads Nucleic Acid Purification Support Center.