PrioCHECK™ Bovine BVDV Ab Plate Kit

Note: The name of this product has changed. It was previously called PRIOCHECK BVDV AB ELISA.

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The PrioCHECK™ BVDV Antibody ELISA is a valuable tool in large-scale screening and eradication programs. The test can detect infected animals in a vaccinated population. The PrioCHECK™ BVDV Antibody ELISA detects antibodies against different subtypes of BVDV (Ia, Ib, and II) in serum, plasma, and bulk milk samples of cattle.

PrioCHECK™ BVDV Antibody ELISA is an inhibition ELISA giving a signal that is reciprocal to the sample antibody concentration. It employs two monoclonal antibodies (mAbs). The two mAbs recognize different epitopes located on the highly conserved NS-3 non-structural protein (p80) of BVDV. One mAb is coated to the plate. The sample is then incubated with the inactivated BVDV antigen in the wells of the coated plate. A second mAb, coupled to an enzyme that generates a color signal, is added. The signal is then measured and when no color is formed, the sample containing anti-BVDV antibodies that have competed for the available antigen is positive for BVDV.