Ambion™ A fluorescently labeled negative control siRNA for the easy monitoring of siRNA uptake by fluorescence microscopy. Purified, annealed, and ready to use, the 5 nmol aliquot is supplied in a stable, dried format.

• Simplifies the monitoring of siRNA delivery
• Functionally tested in several common cell lines
• HPLC purified, duplexed, and ready to use

Visualize siRNA Uptake with Labeled siRNAs
Fluorescent labeled siRNAs allow for the direct observation siRNA cellular uptake, distribution, and localization. Fluorescently labeled siRNAs are commonly used to monitor transfection efficiency during optimization of transfection conditions. In general, siRNAs are rapidly taken up by and distributed throughout cells in as little as 4 hours. Fluorescent labeling of siRNAs allows for rapid monitoring of siRNA delivery by fluorescence microscopy, as opposed to unlabeled siRNAs, whose delivery must be monitored by target gene knockdown. Silencer™ FAM Labeled Negative Control #1 siRNAs provide an easy way to monitor siRNA uptake by chemical transfection. The sequences is identical to that of the Ambion Silencer™ Negative Control# 1 siRNA (SKU #AM4611), but with a fluorescein derivative FAM (5'-carboxyfluorescein; excitation max. = 494 nm, emission max. = 520 nm) on the 5' end of the sense strand, making it highly fluorescent when excited by the appropriate wavelength of light.

Accessory Products:
For the monitoring of siRNA delivery by electroporation, Silencer™ Cy™3 Labeled Negative Control #1 siRNA (SKU #AM4621) is recommended because of its brighter fluorescence signal visible over a broader pH range. Electroporation produces a more diffuse siRNA distribution pattern, resulting in a less intense fluorescence signal than with chemical transfection. Silencer™ control siRNAs are unmodified 21-mers; they are designed to be used with Silencer™ siRNAs, BLOCK-iT™ siRNAs, and other unmodified 21mer siRNAs. If you are using other siRNA products, such as Ambion™ Silencer™ Select siRNAs or STEALTH RNAi™ siRNAs, we recommend that you use control siRNAs designed for use with those siRNAs for more reliable experimental results.