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Citations & References (6)
Invitrogen™
BacLight™ RedoxSensor™ CTC Vitality Kit
The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixationRead more
| Catalog Number | Quantity |
|---|---|
| B34956 | 1 Kit |
Catalog number B34956
Price (HKD)
5,570.00
Each
Quantity:
1 Kit
Price (HKD)
5,570.00
Each
The BacLight™ RedoxSensor™ CTC Vitality Kit provides effective reagents for evaluating bacterial cell health and vitality that can withstand fixation procedures. Briefly, healthy cells respiring via the electron transport chain will absorb and reduce CTC into an insoluble, red fluorescent formazan product. Cells not respiring or respiring at slower rates will reduce less CTC and consequently produce less fluorescent product, giving a semi-quantitative estimate of healthy vs. unhealthy bacteria. Green- and blue-fluorescent nucleic acid stains are included as counterstains to assist differentiation of cells from debris and calculating total cell numbers.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeBacteria
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
FormatTube(s), Slide(s)
Quantity1 Kit
Shipping ConditionRoom Temperature
SolubilityDMSO (Dimethylsulfoxide)
Emission488
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
Product LineBacLight, RedoxSensor
Product TypeCTC Vitality Kit
Unit SizeEach
Contents & Storage
• 5-cyano-2,3-ditolyl tetrazolium chloride (CTC), 5 x 15 mg vials
• SYTO™ 24 green (100 μL in DMSO), 1 vial
• DAPI (5 mg/mL solution in water), 100 μL
Store in freezer (-5°C to -30°C) and protect from light.
• SYTO™ 24 green (100 μL in DMSO), 1 vial
• DAPI (5 mg/mL solution in water), 100 μL
Store in freezer (-5°C to -30°C) and protect from light.
Frequently asked questions (FAQs)
What bacterial parameters can I look at by flow cytometry?
Citations & References (6)
Citations & References
Abstract
Flow cytometric analysis of 5-cyano-2,3-ditolyl tetrazolium chloride activity of marine bacterioplankton in dilution cultures.
Journal:Appl Environ Microbiol
PubMed ID:10347021
'The respiratory activity of marine bacteria is an important indication of the ecological functioning of these organisms in marine ecosystems. The redox dye 5-cyano-2,3-ditolyl tetrazolium chloride (CTC) is reduced intracellularly in respiring cells to an insoluble, fluorescent precipitate. This product is detectable and quantifiable by flow cytometry in individual cells.
Comparison of the antimicrobial effects of chlorine, silver ion, and tobramycin on biofilm.
Journal:Antimicrob Agents Chemother
PubMed ID:18195062
'The systematic understanding of how various antimicrobial agents are involved in controlling biofilms is essential in order to establish an effective strategy for biofilm control, since many antimicrobial agents are effective against planktonic cells but are ineffective when they are used against the same bacteria growing in a biofilm state.
Characterization of osmotically induced filaments of Salmonella enterica.
Journal:Appl Environ Microbiol
PubMed ID:22798362
'Salmonella enterica forms aseptate filaments with multiple nucleoids when cultured in hyperosmotic conditions. These osmotic-induced filaments are viable and form single colonies on agar plates even though they contain multiple genomes and have the potential to divide into multiple daughter cells. Introducing filaments that are formed during osmotic stress into
Inactivation of Escherichia coli by nanoparticulate zerovalent iron and ferrous ion.
Journal:Appl Environ Microbiol
PubMed ID:20870787
'The mechanism of Escherichia coli inactivation by nanoparticulate zerovalent iron (nZVI) and Fe(II) was investigated using reactive oxygen species (ROS) quenchers and probes, an oxidative stress assay, and microscopic observations. Disruption of cell membrane integrity and respiratory activity was observed under deaerated conditions [more disruption by nZVI than Fe(II)], and
How the insect immune system interacts with an obligate symbiotic bacterium.
Journal:Proc Biol Sci
PubMed ID:20719775
The animal immune system provides defence against microbial infection, and the evolution of certain animal-microbial symbioses is predicted to involve adaptive changes in the host immune system to accommodate the microbial partner. For example, the reduced humoral immune system in the pea aphid Acyrthosiphon pisum, including an apparently non-functional immune