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Citations & References (18)
Invitrogen™
BTC, AM, cell permeant
BTC, AM is a cell-permeant coumarin benzothiazole-based Ca2+ indicator. It exhibits a shift in excitation maximum from about 480 nmRead more
| Catalog Number | Quantity |
|---|---|
| B6791 | 100 μg |
Catalog number B6791
Price (HKD)
3,269.00
Each
Quantity:
100 μg
Price (HKD)
3,269.00
Each
BTC, AM is a cell-permeant coumarin benzothiazole-based Ca2+ indicator. It exhibits a shift in excitation maximum from about 480 nm to 400 nm upon binding Ca2+, which permits ratiometric measurements that are essentially independent of uneven dye loading cell thickness, photobleaching and dye leakage. BTC has high selectivity for Ca2+ and a Kd ∼7 μM enabling quantitation of high intracellular Ca2+ levels.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Quantity100 μg
Shipping ConditionRoom Temperature
For Use With (Application)Cell Viability and Proliferation
For Use With (Equipment)Flow Cytometer
Product TypeCalcium Indicator
Unit SizeEach
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.
Citations & References (18)
Citations & References
Abstract
AM-loading of fluorescent Ca2+ indicators into intact single fibers of frog muscle.
Journal:Biophys J
PubMed ID:9168048
'The AM loading of a number of different fluorescent Ca2+ indicators was compared in intact single fibers of frog muscle. Among the 13 indicators studied, loading rates (the average increase in the fiber concentration of indicator per first 60 min of loading) varied approximately 100-fold, from approximately 3 microM/h to
Significance of Na/Ca exchange for Ca2+ buffering and electrical activity in mouse pancreatic beta-cells.
Journal:Biophys J
PubMed ID:10096898
'We have combined the patch-clamp technique with microfluorimetry of the cytoplasmic Ca2+ concentration ([Ca2+]i) to characterize Na/Ca exchange in mouse beta-cells and to determine its importance for [Ca2+]i buffering and shaping of glucose-induced electrical activity. The exchanger contributes to Ca2+ removal at [Ca2+]i above 1 microM, where it accounts for
Photolysis-induced suppression of inhibition in rat hippocampal CA1 pyramidal neurons.
Journal:J Physiol
PubMed ID:11410632
'1. Whole cell patch clamp recording, Ca(2+) measurement with ratiometric fluorescent dyes and photolysis of caged Ca(2+) were combined to investigate the depolarization- and photolysis-induced suppression of inhibition (DSI and PSI) in rat hippocampal CA1 pyramidal cells. 2. A 5-s depolarization from -70 mV to 0 mV or a 6-s
Ionized intracellular calcium concentration predicts excitotoxic neuronal death: observations with low-affinity fluorescent calcium indicators.
Journal:J Neurosci
PubMed ID:9254679
'Cytosolic calcium ([Ca2+]i) is an important mediator of neuronal signal transduction, participating in diverse biochemical reactions that elicit changes in synaptic efficacy, metabolic rate, and gene transcription. Excessive [Ca2+]i also has been implicated as a cause of acute neuronal injury, although measurement of [Ca2+]i in living neurons by fluorescent calcium
Ionic selectivity of low-affinity ratiometric calcium indicators: mag-Fura-2, Fura-2FF and BTC.
Journal:Cell Calcium
PubMed ID:10756974
'Accurate measurement of elevated intracellular calcium levels requires indicators with low calcium affinity and high selectivity. We examined fluorescence spectral properties and ionic specificity of three low-affinity, ratiometric indicators structurally related to Fura-2: mag-Fura-2 (furaptra), Fura-2FF, and BTC. The indicators differed in respect to their excitation wavelengths, affinity for Ca2+