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Citations & References (38)
Invitrogen™
Image-iT™ Lipid Peroxidation Kit, for live cell analysis
The Image-iT™ Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11Read more
| Catalog Number | Quantity |
|---|---|
| C10445 | 1 Kit |
Catalog number C10445
Price (HKD)
4,305.00
Each
Quantity:
1 Kit
Price (HKD)
4,305.00
Each
The Image-iT™ Lipid Peroxidation Kit enables the detection of lipid peroxidation in live cells through oxidation of BODIPY™ 581/591 C11 reagent. This reagent localizes to membranes throughout live cells and upon oxidation by lipid hydroperoxides, displays a shift in peak fluorescence emission from ∼590 nm to ∼510 nm. Fluorescence from live cells shifts from red to green, providing a ratiometric indication of lipid peroxidation compatible with traditional and high content microscopy, as well as flow cytometry. This kit also includes cumene hydroperoxide as a positive control compound to induce lipid peroxidation in cells.
Additional Information:
• The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Additional Information:
• The reagent is provided as a stable, ready-to-use DMSO solution with a simple protocol compatible with standard workflows in fluorescence microscopy
• The reagent is provided as five single-use vials containing sufficient total reagent for five 96-well plates or 100 coverslips
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorGreen
FormatFrozen
Quantity1 Kit
Product LineImage-iT
Product TypeLipid Sensor Kit
Unit SizeEach
Citations & References (38)
Citations & References
Abstract
Immuno-spin trapping of heme-induced protein radicals: Implications for heme oxygenase-1 induction and heme degradation.
Journal:Free Radic Biol Med
PubMed ID:23624303
'Heme, in the presence of hydrogen peroxide, can act as a peroxidase. Intravascular hemolysis results in a massive release of heme into the plasma in several pathophysiological conditions such as hemolytic anemia, malaria, and sickle cell disease. Heme is known to induce heme oxygenase-1(HO-1) expression, and the extent of induction
Cadmium disorganises the scaffolding of gap and tight junction proteins in the hepatic cell line WIF B9.
Journal:
PubMed ID:24117459
'Hepatocytes, which perform the main functions of the liver, are particularly vulnerable to toxic agents such as cadmium, an environmental pollutant. To identify the molecular targets for cadmium in hepatocytes, we have studied the effects of CdCl2 on the hybrid cell line WIF-B9 that exhibits stable structural and functional hepatocytic
Use of fluorogenic probes to differentiate between hydrophilic and lipophilic antioxidant activity in a fish cell line.
Journal:J Agric Food Chem
PubMed ID:22136585
'In finfish aquaculture, dietary antioxidants have been shown to improve indicators of general fish health and to inhibit the oxidative deterioration of polyunsaturated fatty acids. To facilitate the characterization of novel antioxidants or antioxidant mixtures, we developed assays for antioxidant activity in a fish cell line. We used 2'',7''-dichlorodihydrofluorescein diacetate
Temperature acclimation alters oxidative capacities and composition of membrane lipids without influencing activities of enzymatic antioxidants or susceptibility to lipid peroxidation in fish muscle.
Journal:J Exp Biol
PubMed ID:20086129
'Cold acclimation of ectotherms results typically in enhanced oxidative capacities and lipid remodeling, changes that should increase the risk of lipid peroxidation (LPO). It is unclear whether activities of antioxidant enzymes may respond in a manner to mitigate the increased potential for LPO. The current study addresses these questions using
Supra-physiological doses of testosterone affect membrane oxidation of human neutrophils monitored by the fluorescent probe C(11)-BODIPY (581/591).
Journal:Eur J Appl Physiol
PubMed ID:23160653
'The purpose of this study was to determine the effects of supra-physiological doses of testosterone (TES) on membrane oxidation of activated human neutrophils in vitro using an innovative and sensitive technique: the real-time detection with the fluorescence probe C(11)-BODIPY(581/591). Methodological controls were performed with the lipid-soluble and powerful antioxidant astaxanthin